CoraLite® Plus 647 Anti-Human CD20 (2H7) Mouse IgG2a Recombinant Antibody

CD20 Recombinant Antibody for FC

Host / Isotype

Mouse / IgG2a

Reactivity

human

Applications

FC

Conjugate

CoraLite® Plus 647 Fluorescent Dye

CloneNo.

2H7

Cat no : CL647-65575

Synonyms

Bp35, B-lymphocyte surface antigen B1, B-lymphocyte antigen CD20, B1, B lymphocyte antigen CD20

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Validation Data Gallery

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  FC experiment of human PBMCs using CL647-65575

  1x10^6 human PBMCs were surface stained with CoraLite® Plus 488 Anti-Human CD19 and 5 ul CoraLite® Plus 647 Anti-Human CD20 Mouse IgG2a Recombinant Antibody (CL647-65575, Clone: 2H7), or CoraLite® Plus 647 Mouse IgG2a Isotype Control (CL647-65208, Clone: C1.18.4). Cells were not fixed. Lymphocytes were gated.

• 

  FC experiment of human PBMCs using CL647-65575

  1x10^6 human PBMCs were surface stained with 5 ul CoraLite® Plus 647 Anti-Human CD20 Mouse IgG2a Recombinant Antibody (CL647-65575, Clone: 2H7) (red), or CoraLite® Plus 647 Mouse IgG2a Isotype Control (CL647-65208, Clone: C1.18.4) (blue). Cells were not fixed. Lymphocytes were gated.

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Tested Applications

• Positive FC detected in: human PBMCs

Recommended dilution

Application	Dilution
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 µl per 10^6 cells in a 100 µl suspension or 5 µl per 100 µl of whole blood.
Sample-dependent, Check data in validation data gallery.

Product Information

CL647-65575 targets CD20 in FC applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG2a
• Class: Recombinant
• Type: Antibody
• Immunogen: Human tonsillar B cells
• Full Name: membrane-spanning 4-domains, subfamily A, member 1
• Calculated Molecular Weight: 297 aa, 33 kDa
• GenBank Accession Number: BC002807
• Gene Symbol: CD20
• Gene ID (NCBI): 931
• Conjugate: CoraLite® Plus 647 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 654 nm / 674 nm
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.09% sodium azide and 0.5% BSA.
• Storage Conditions: Store at 2-8°C. Avoid exposure to light.

Background Information

CD20 is a 33-37 kDa transmembrane phosphoprotein belonging to the membrane-spanning 4A family (PMID: 3260267; 16785532). CD20 is a B-lymphocyte surface molecule that is widely expressed during B-cell ontogeny, from early pre-B-cell developmental stages until final differentiation into plasma cells (PMID: 7524522). CD20 functions as calcium-permeable cation channel (PMID: 7684739). It is involved in the regulation of B-cell activation, proliferation and differentiation (PMID: 7524522).