Biotin Anti-Human CD20 (2H7) Mouse IgG2a Recombinant Antibody

CD20 Uni-rAb^TM Recombinant Antibody for FC

Host / Isotype

Mouse / IgG2a

Reactivity

human

Applications

FC

Conjugate

Biotin

CloneNo.

2H7

Cat no : Biotin-65575

Synonyms

Bp35, B-lymphocyte surface antigen B1, B-lymphocyte antigen CD20, B1, B lymphocyte antigen CD20

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Validation Data Gallery

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  FC experiment of human PBMCs using Biotin-65575

  1x10^6 human PBMCs were surface stained with 0.25 ug Biotin Anti-Human CD20 (2H7) Mouse IgG2a Recombinant Antibody (Biotin-65575, Clone: 2H7) and CoraLite®488-conjugated streptavidin, or unstained. Cells were co-stained with CoraLite® Plus 647 Anti-Human CD19. Cells were not fixed. Lymphocytes were gated.

• 

  FC experiment of human PBMCs using Biotin-65575

  1x10^6 human PBMCs were surface stained with 0.25 ug Biotin Anti-Human CD20 (2H7) Mouse IgG2a Recombinant Antibody (Biotin-65575, Clone: 2H7) and CoraLite®488-conjugated streptavidin (red), or unstained (blue). Cells were not fixed. Lymphocytes were gated.

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Tested Applications

• Positive FC detected in: human PBMCs

Recommended dilution

Application	Dilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

Biotin-65575 targets CD20 in FC applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG2a
• Class: Recombinant
• Type: Antibody
• Immunogen: Human tonsillar B cells
• Full Name: membrane-spanning 4-domains, subfamily A, member 1
• Calculated Molecular Weight: 297 aa, 33 kDa
• GenBank Accession Number: BC002807
• Gene Symbol: CD20
• Gene ID (NCBI): 931
• Conjugate: Biotin
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.09% sodium azide.
• Storage Conditions: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

CD20 is a 33-37 kDa transmembrane phosphoprotein belonging to the membrane-spanning 4A family (PMID: 3260267; 16785532). CD20 is a B-lymphocyte surface molecule that is widely expressed during B-cell ontogeny, from early pre-B-cell developmental stages until final differentiation into plasma cells (PMID: 7524522). CD20 functions as calcium-permeable cation channel (PMID: 7684739). It is involved in the regulation of B-cell activation, proliferation and differentiation (PMID: 7524522).