PE Anti-Human CD1a (HI149)

CD1a Monoclonal Antibody for FC

Host / Isotype

Mouse / Mouse IgG1

Reactivity

Human

Applications

FC

Conjugate

PE Fluorescent Dye

CloneNo.

HI149

Cat no : PE-65172

Synonyms

CD1, CD1A, CD1a molecule, FCB6, HTA1, hTa1 thymocyte antigen, R4, T6

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Validation Data Gallery

• 

  FC experiment of MOLT‑4 using PE-65172

  1X10^6 MOLT-4 cells were surface stained with 20.00 ul/test PE-Anti-Human CD1a antibody (PE-65172, Clone: HI149) (red) or PE-mouse IgG1 isotype control (blue). Cells were not fixed.

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Tested Applications

• Positive FC detected in: MOLT‑4

Recommended dilution

Application	Dilution
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 20 µl per 10^6 cells in a 100 µl suspension or 20 µl per 100 µl of whole blood.
Sample-dependent, Check data in validation data gallery.

Product Information

PE-65172 targets CD1a in FC applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Host / Isotype: Mouse / Mouse IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: Human thymocytes
• Full Name: CD1a molecule
• Calculated Molecular Weight: 327 aa, 37 kDa
• GenBank Accession Number: BC031645
• Gene Symbol: CD1A
• Gene ID (NCBI): 909
• RRID: AB_2883947
• Conjugate: PE Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 496 nm, 565 nm / 578 nm
• Form: Liquid
• Purification Method: The purified antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. The conjugate is purified by size-exclusion chromatography.
• Storage Buffer: PBS with 0.1% sodium azide.
• Storage Conditions: Store at 2-8°C. Avoid exposure to light.