Anti-Human CD1a (HI149)

CD1a Monoclonal Antibody for FC

Host / Isotype

Mouse / IgG1, kappa

Reactivity

human

Applications

FC

Conjugate

Unconjugated

CloneNo.

HI149

Cat no : 65172-1-Ig

Synonyms

hTa1 thymocyte antigen, HTA1, HI149, FCB6, CD1a molecule

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Validation Data Gallery

• 

  FC experiment of MOLT-4 using 65172-1-Ig

  0.5x10^6 MOLT-4 cells were surface stained with 0.2 ug Anti-Human CD1a (65172-1-Ig, Clone: HI149) (red) or Mouse IgG1 Isotype Control (65124-1-Ig, Clone: MOPC-21) (blue), and Multi-rAb CoraLite ® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002). Cells were not fixed.

• 

  FC experiment of MOLT-4 using 65172-1-Ig

  0.5x10^6 MOLT-4 cells were surface stained with 0.2 ug Anti-Human CD1a (65172-1-Ig, Clone: HI149) or Mouse IgG1 Isotype Control (65124-1-Ig, Clone: MOPC-21), and Multi-rAb CoraLite ® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002). Cells were not fixed.

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Tested Applications

• Positive FC detected in: MOLT-4 cells

Recommended dilution

Application	Dilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

65172-1-Ig targets CD1a in FC applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG1, kappa
• Class: Monoclonal
• Type: Antibody
• Immunogen: Human thymocytes
• Full Name: CD1a molecule
• Calculated Molecular Weight: 327 aa, 37 kDa
• GenBank Accession Number: BC031645
• Gene Symbol: CD1A
• Gene ID (NCBI): 909
• RRID: AB_2918448
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Purified by protein-A affinity chromatography
• Storage Buffer: PBS with 0.09% sodium azide.
• Storage Conditions: Store at 2-8°C. Stable for one year after shipment.

Background Information

CD1a is an antigen-presenting protein that binds self and non-self lipid and glycolipid antigens and presents them to T-cell receptors on natural killer T-cells. During protein synthesis and maturation, CD1 family members bind endogenous lipids that are replaced by lipid or glycolipid antigens when the proteins are internalized and pass through endosomes, before trafficking back to the cell surface.