Mouse CD19 Recombinant antibody, PBS Only (Capture/Detector)

CD19 Recombinant Antibody for WB, Indirect ELISA, Cytometric bead array

Host / Isotype

Rabbit / IgG

Reactivity

Mouse

Applications

WB, Indirect ELISA, Cytometric bead array

Conjugate

Unconjugated

CloneNo.

240135A3

Cat no : 83254-1-PBS

Synonyms

AW495831, Cd19, CD19 antigen

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Validation Data Gallery

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  WB analysis using 83254-1-RR (same clone as 83254-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 83254-1-RR (CD19 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83254-1-PBS in a different storage buffer formulation.

• 

  Cytometric bead array standard curve of MP00253-1

  Cytometric bead array standard curve ofMP00253-1, Mouse CD19 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83254-2-PBS. Detection antibody: 83254-1-PBS. Standard: Eg0907. Range: 2.5-80 ng/mL.

• 

  Cytometric bead array standard curve of MP00253-3

  Cytometric bead array standard curve ofMP00253-3, Mouse CD19 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83254-1-PBS. Detection antibody: 83254-4-PBS. Standard: Eg0907. Range: 5-80 ng/mL.

• 

  Affinity experiments of 83254-1-RR

  Biolayer interferometry (BLl) kinetic assays of 83254-1-RR against Mouse CD19 were performed. The affinity constant is 0.303 nM.

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Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

83254-1-PBS targets CD19 as part of a matched antibody pair:

MP00253-1: 83254-2-PBS capture and 83254-1-PBS detection (validated in Cytometric bead array)

MP00253-3: 83254-1-PBS capture and 83254-4-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: Mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Recombinant protein
• Full Name: CD19 antigen
• Calculated Molecular Weight: 60 kDa
• GenBank Accession Number: NM_009844.2
• Gene Symbol: Cd19
• Gene ID (NCBI): 12478
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.