CD19 Recombinant antibody

CD19 Uni-rAb^TM Recombinant Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Mouse, Rat

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

240135A3

Cat no : 83254-1-RR

Synonyms

AW495831, Cd19, CD19 antigen

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Validation Data Gallery

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  WB analysis using 83254-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 83254-1-RR (CD19 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  Affinity and Kinetic Characterization of 83254-1-RR

  Biolayer interferometry (BLl) kinetic assays of 83254-1-RR against Mouse CD19 were performed. The affinity constant is 0.303 nM.

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Tested Applications

• Positive WB detected in: mouse spleen tissue, rat spleen tissue

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

83254-1-RR targets CD19 in WB, ELISA applications and shows reactivity with Mouse, Rat samples.

• Tested Reactivity: Mouse, Rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Recombinant protein
• Full Name: CD19 antigen
• Calculated Molecular Weight: 60 kDa
• Observed Molecular Weight: 62-70 kDa
• GenBank Accession Number: NM_009844.2
• Gene Symbol: Cd19
• Gene ID (NCBI): 12478
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CD19 is a type I transmembrane glycoprotein belonging to the immunoglobulin superfamily (PMID: 2472450). It is expressed by B cells and follicular dendritic cells. CD19 is up-regulated at the step of B-lineage commitment during the differentiation of the hematopoietic stem cell, it remains on during subsequent stages of differentiation until finally down-regulated during terminal differentiation into plasma cells (PMID: 8528044). CD19 is involved in B cell development, activation and differentiation. It is the dominant component for the signaling complex on B cells that includes CD21 (CR2), CD81 (TAPA-1) and CD225 and acts as a critical co-receptor for BCR signal transduction (PMID: 23210908).