Anti-Human CD19 (4G7) Mouse IgG2a Recombinant Antibody

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  FC experiment of human PBMCs using 65562-1-MR

  1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human CD19 (4G7) Mouse IgG2a Recombinant Antibody (65562-1-MR, Clone: 4G7) or Mouse IgG2a Isotype Control (C1.18.4) (65208-1-Ig, Clone: C1.18.4) and PE-conjugated Goat Anti-Mouse IgG. Cells were co-stained with APC Anti-Human CD3 (OKT3) Mouse IgG2a Recombinant Antibody (APC-65569, Clone: OKT3). Cells were not fixed. Lymphocytes were gated.

• 

  FC experiment of human PBMCs using 65562-1-MR

  1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human CD19 (4G7) Mouse IgG2a Recombinant Antibody (65562-1-MR, Clone: 4G7) (red) or Mouse IgG2a Isotype Control (C1.18.4) (65208-1-Ig, Clone: C1.18.4) (blue), and PE-conjugated Goat Anti-Mouse IgG. Cells were not fixed. Lymphocytes were gated.

CD19 Uni-rAb^TM Recombinant Antibody for FC

Cat No. 65562-1-MR

Clone No.4G7

Host / Isotype

Mouse / IgG2a

Reactivity

human

Applications

FC

Synonyms

CD19 molecule, B-lymphocyte surface antigen B4, B-lymphocyte antigen CD19, B4, B lymphocyte antigen CD19

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Tested Applications

• Positive FC detected in: human PBMCs

Recommended dilution

Application	Dilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

65562-1-MR targets CD19 in FC applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG2a
• Class: Recombinant
• Type: Antibody
• Immunogen: Human chronic lymphocytic leukemia (CLL) cells
• Full Name: CD19 molecule
• Calculated Molecular Weight: 556 aa, 61 kDa
• GenBank Accession Number: BC006338
• Gene Symbol: CD19
• Gene ID (NCBI): 930
• ENSEMBL Gene ID: ENSG00000177455
• RRID: AB_3670316
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: P15391
• Storage Buffer: PBS with 0.09% sodium azide.
• Storage Conditions: Store at 2-8°C. Stable for one year after shipment.

Background Information

CD19 is a 95 kDa type I transmembrane glycoprotein belonging to the immunoglobulin superfamily (PMID: 2472450). It is expressed by B cells and follicular dendritic cells. CD19 is up-regulated at the step of B-lineage commitment during the differentiation of the hematopoietic stem cell, it remains on during subsequent stages of differentiation until finally down-regulated during terminal differentiation into plasma cells (PMID: 8528044). CD19 is involved in B cell development, activation and differentiation. It is the dominant component for the signaling complex on B cells that includes CD21 (CR2), CD81 (TAPA-1) and CD225 and acts as a critical co-receptor for BCR signal transduction (PMID: 23210908).