CoraLite® Plus 405 Anti-Human CD19 (4G7) Mouse IgG2a Recombinant Antibody
CD19 Recombinant Antibody for FC
Host / Isotype
Mouse / IgG2a
Reactivity
human
Applications
FC
Conjugate
CoraLite® Plus 405 Fluorescent Dye
CloneNo.
4G7
Cat no : CL405-65562
Synonyms
Validation Data Gallery
Tested Applications
Positive FC detected in | human PBMCs |
Recommended dilution
Application | Dilution |
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This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 µl per 10^6 cells in a 100 µl suspension or 5 µl per 100 µl of whole blood. | |
Sample-dependent, Check data in validation data gallery. |
Product Information
CL405-65562 targets CD19 in FC applications and shows reactivity with human samples.
Tested Reactivity | human |
Host / Isotype | Mouse / IgG2a |
Class | Recombinant |
Type | Antibody |
Immunogen | Human chronic lymphocytic leukemia (CLL) cells |
Full Name | CD19 molecule |
Calculated Molecular Weight | 556 aa, 61 kDa |
GenBank Accession Number | BC006338 |
Gene Symbol | CD19 |
Gene ID (NCBI) | 930 |
Conjugate | CoraLite® Plus 405 Fluorescent Dye |
Excitation/Emission Maxima Wavelengths | 399 nm / 422 nm |
Form | Liquid |
Purification Method | Protein A purification |
Storage Buffer | PBS with 0.09% sodium azide and 0.5% BSA. |
Storage Conditions | Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment. |
Background Information
CD19 is a 95 kDa type I transmembrane glycoprotein belonging to the immunoglobulin superfamily (PMID: 2472450). It is expressed by B cells and follicular dendritic cells. CD19 is up-regulated at the step of B-lineage commitment during the differentiation of the hematopoietic stem cell, it remains on during subsequent stages of differentiation until finally down-regulated during terminal differentiation into plasma cells (PMID: 8528044). CD19 is involved in B cell development, activation and differentiation. It is the dominant component for the signaling complex on B cells that includes CD21 (CR2), CD81 (TAPA-1) and CD225 and acts as a critical co-receptor for BCR signal transduction (PMID: 23210908).
Protocols
Product Specific Protocols | |
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FC protocol for CL Plus 405 CD19 antibody CL405-65562 | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |