CoraLite® Plus 405 Anti-Human CD19 (4G7) Mouse IgG2a Recombinant Antibody

CD19 Uni-rAb^TM Recombinant Antibody for FC

Host / Isotype

Mouse / IgG2a

Reactivity

human

Applications

FC

Conjugate

CoraLite® Plus 405 Fluorescent Dye

CloneNo.

4G7

Cat no : CL405-65562

Synonyms

CD19 molecule, B-lymphocyte surface antigen B4, B-lymphocyte antigen CD19, B4, B lymphocyte antigen CD19

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Validation Data Gallery

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  FC experiment of human PBMCs using CL405-65562

  1x10^6 human PBMCs were surface stained with APC Anti-Human CD3, and 5 ul CoraLite® Plus 405 Anti-Human CD19 (4G7) Mouse IgG2a Recombinant Antibody (CL405-65562, Clone:4G7) (right), or CoraLite® Plus 405 Mouse IgG2a Isotype Control (C1.18.4) (CL405-65208, Clone: C1.18.4) (left). Cells were not fixed. Lymphocytes were gated.

• 

  FC experiment of human PBMCs using CL405-65562

  1x10^6 human PBMCs were surface stained with 5 ul CoraLite® Plus 405 Anti-Human CD19 (4G7) Mouse IgG2a Recombinant Antibody (CL405-65562, Clone:4G7) (red), or CoraLite® Plus 405 Mouse IgG2a Isotype Control (C1.18.4) (CL405-65208, Clone: C1.18.4) (blue). Cells were not fixed. Lymphocytes were gated.

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Tested Applications

• Positive FC detected in: human PBMCs

Recommended dilution

Application	Dilution
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 µl per 10^6 cells in a 100 µl suspension or 5 µl per 100 µl of whole blood.
Sample-dependent, Check data in validation data gallery.

Product Information

CL405-65562 targets CD19 in FC applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG2a
• Class: Recombinant
• Type: Antibody
• Immunogen: Human chronic lymphocytic leukemia (CLL) cells
• Full Name: CD19 molecule
• Calculated Molecular Weight: 556 aa, 61 kDa
• GenBank Accession Number: BC006338
• Gene Symbol: CD19
• Gene ID (NCBI): 930
• Conjugate: CoraLite® Plus 405 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 399 nm / 422 nm
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.09% sodium azide and 0.5% BSA.
• Storage Conditions: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

CD19 is a 95 kDa type I transmembrane glycoprotein belonging to the immunoglobulin superfamily (PMID: 2472450). It is expressed by B cells and follicular dendritic cells. CD19 is up-regulated at the step of B-lineage commitment during the differentiation of the hematopoietic stem cell, it remains on during subsequent stages of differentiation until finally down-regulated during terminal differentiation into plasma cells (PMID: 8528044). CD19 is involved in B cell development, activation and differentiation. It is the dominant component for the signaling complex on B cells that includes CD21 (CR2), CD81 (TAPA-1) and CD225 and acts as a critical co-receptor for BCR signal transduction (PMID: 23210908).