Anti-Human CD19 (4G7) Mouse IgG2a Recombinant Antibody, PBS Only

• 

  FC experiment of human PBMCs using 65562-1-MR (same clone as 65562-1-PBS)

  1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human CD19 (4G7) Mouse IgG2a RecAb (65562-1-MR, Clone: 4G7) or Mouse IgG2a Isotype Control (C1.18.4) (65208-1-Ig, Clone: C1.18.4), and PE-conjugated Goat Anti-Mouse IgG. Cells were then stained with CoraLite® Plus 647 Anti-Human CD3. Cells were not fixed. Lymphocytes were gated. This data was developed using the same antibody clone with 65562-1-PBS in a different storage buffer formulation.

• 

  FC experiment of human PBMCs using 65562-1-MR (same clone as 65562-1-PBS)

  1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human CD19 (4G7) Mouse IgG2a RecAb (65562-1-MR, Clone: 4G7) (red) or Mouse IgG2a Isotype Control (C1.18.4) (65208-1-Ig, Clone: C1.18.4) (blue), and PE-conjugated Goat Anti-Mouse IgG. Cells were not fixed. Lymphocytes were gated. This data was developed using the same antibody clone with 65562-1-PBS in a different storage buffer formulation.

CD19 Uni-rAb^® Recombinant Antibody for FC

Cat No. 65562-1-PBS

Clone No.4G7

Host / Isotype

Mouse / IgG2a

Reactivity

human

Applications

FC

Synonyms

4G7, B lymphocyte antigen CD19, B4, B-lymphocyte antigen CD19, B-lymphocyte surface antigen B4

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Product Information

65562-1-PBS targets CD19 in FC applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG2a
• Class: Recombinant
• Type: Antibody
• Immunogen:

  Human chronic lymphocytic leukemia (CLL) cells

• Full Name: CD19 molecule
• Calculated Molecular Weight: 556 aa, 61 kDa
• GenBank Accession Number: BC006338
• Gene Symbol: CD19
• Gene ID (NCBI): 930
• ENSEMBL Gene ID: ENSG00000177455
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: P15391
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C. Stable for one year after shipment.

Background Information

CD19 is a 95 kDa type I transmembrane glycoprotein belonging to the immunoglobulin superfamily (PMID: 2472450). It is expressed by B cells and follicular dendritic cells. CD19 is up-regulated at the step of B-lineage commitment during the differentiation of the hematopoietic stem cell, it remains on during subsequent stages of differentiation until finally down-regulated during terminal differentiation into plasma cells (PMID: 8528044). CD19 is involved in B cell development, activation and differentiation. It is the dominant component for the signaling complex on B cells that includes CD21 (CR2), CD81 (TAPA-1) and CD225 and acts as a critical co-receptor for BCR signal transduction (PMID: 23210908).