Anti-Human CD18 (TS1/18) Mouse IgG1 Recombinant Antibody

CD18 Uni-rAb^TM Recombinant Antibody for FC

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

FC

Conjugate

Unconjugated

CloneNo.

TS1/18

Cat no : 65524-1-MR

Synonyms

CD18, Integrin beta 2, ITGB2, LAD, LCAMB, LFA 1, MAC 1, MF17, MFI7

---

Validation Data Gallery

• 

  FC experiment of human PBMCs using 65524-1-MR

  1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human CD18 (65524-1-MR, Clone:TS1/18) or 0.25 ug Mouse IgG1 Isotype Control (MOPC-21) (65124-1-Ig, Clone: MOPC-21), and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). Cells were not fixed.

---

Tested Applications

• Positive FC detected in: human PBMCs

Recommended dilution

Application	Dilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

65524-1-MR targets CD18 in FC applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Host / Isotype: Mouse / IgG1
• Class: Recombinant
• Type: Antibody
• Immunogen: N/A
• Full Name: integrin, beta 2 (complement component 3 receptor 3 and 4 subunit)
• Calculated Molecular Weight: 85 kDa
• GenBank Accession Number: BC005861
• Gene Symbol: CD18
• Gene ID (NCBI): 3689
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Affinity purification
• Storage Buffer: PBS with 0.09% sodium azide.
• Storage Conditions: Store at 2-8°C. Stable for one year after shipment.

Background Information

CD18, also known as integrin beta-2, is a transmembrane protein that forms heterodimers with CD11a, CD11b, CD11c, CD11d (PMID: 1968349). CD11a/CD18 (LFA-1) is expressed by all leukocytes, while CD11b/CD18 (Mac-1) and CD11c/CD18 (p150,95) are normally restricted to expression on monocytes, macrophages, polymorphonuclear lymphocytes (PMNs), and natural killer cells (PMID: 1968349; 3109455; 10946284). CD18 plays an important role in mediating cell adhesion during immune response and defects of CD18 cause leukocyte adhesion deficiency (PMID: 3594570).