Anti-Human CD163 (GHI/61) Mouse IgG2a Recombinant Antibody, PBS Only

CD163 Uni-rAb^TM Recombinant Antibody for FC

Host / Isotype

Mouse / IgG2a

Reactivity

human

Applications

FC

Conjugate

Unconjugated

CloneNo.

GHI/61

Cat no : 65561-1-PBS

Synonyms

Scavenger receptor cysteine-rich type 1 protein M130, MM130, M130, Hemoglobin scavenger receptor, GHI/61

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Validation Data Gallery

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  FC experiment of human PBMCs using 65561-1-MR (same clone as 65561-1-PBS)

  1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human CD163 Mouse IgG2a Recombinant Antibody (65561-1-MR, Clone:GHI/61) or Mouse IgG2a Isotype Control (65208-1-Ig, Clone: C1.18.4), and CoraLite®488-Conjugated AffiniPure Donkey Anti-Mouse IgG(H+L). Cells were then stained with CoraLite® Plus 647 Anti-Human CD11b. Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed. Monocytes were gated. This data was developed using the same antibody clone with 65561-1-PBS in a different storage buffer formulation.

• 

  FC experiment of human PBMCs using 65561-1-MR (same clone as 65561-1-PBS)

  1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human CD163 Mouse IgG2a Recombinant Antibody (65561-1-MR, Clone:GHI/61) (red) or Mouse IgG2a Isotype Control (65208-1-Ig, Clone: C1.18.4) (blue), and CoraLite®488-Conjugated AffiniPure Donkey Anti-Mouse IgG(H+L). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed. Monocytes were gated. This data was developed using the same antibody clone with 65561-1-PBS in a different storage buffer formulation.

• 

  FC experiment of human PBMCs using 65561-1-MR (same clone as 65561-1-PBS)

  1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human CD163 Mouse IgG2a Recombinant Antibody (65561-1-MR, Clone:GHI/61) (left) or 0.25 ug Anti-Human CD163 (65169-1-Ig, Clone: GHI/61) (right), and CoraLite®488-Conjugated AffiniPure Donkey Anti-Mouse IgG(H+L). Cells were then stained with CoraLite® Plus 647 Anti-Human CD11b. Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed. Monocytes were gated. This data was developed using the same antibody clone with 65561-1-PBS in a different storage buffer formulation.

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Product Information

65561-1-PBS targets CD163 in FC applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG2a
• Class: Recombinant
• Type: Antibody
• Immunogen: Glycoprotein preparation from hairy cell leukemia spleen
• Full Name: CD163 molecule
• Calculated Molecular Weight: 1156 aa, 125 kDa
• GenBank Accession Number: BC051281
• Gene Symbol: CD163
• Gene ID (NCBI): 9332
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C. Stable for one year after shipment.

Background Information

CD163, also known as M130, is a membrane glycoprotein that belongs to the scavenger receptor superfamily (PMID: 8370408). It is an acute phase-regulated and signal-inducing macrophage protein expressed exclusively in monocytes and tissue macrophages (PMID: 11196644). CD163 mediates endocytosis of haptoglobin-haemoglobin complexes (PMID: 11196644). The uptake of haptoglobin by macrophages contributes to the recycling of iron and also to the inflammatory response (PMID: 22900885). Soluble CD163 (sCD163), as a result of ectodomain shedding during inflammatory activation of macrophages, circulates in blood and has been suggested as a plasma/serum marker for macrophage activity (PMID: 12570164).