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Anti-Human CD163 (GHI/61) Mouse IgG2a Recombinant Antibody

CD163 Uni-rAbTM Recombinant Antibody for FC

Host / Isotype

Mouse / IgG2a

Reactivity

Human

Applications

FC

Conjugate

Unconjugated

CloneNo.

GHI/61

Cat no : 65561-1-MR

Synonyms

CD163, CD163 molecule, Hemoglobin scavenger receptor, M130, MM130, sCD163

Validation Data Gallery

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  • Flow cytometry (FC) experiment of human PBMCs using Anti-Human CD163 (GHI/61) Mouse IgG2a Recombinant (65561-1-MR)

    FC experiment of human PBMCs using 65561-1-MR

    1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human CD163 Mouse IgG2a Recombinant Antibody (65561-1-MR, Clone:GHI/61) or Mouse IgG2a Isotype Control (65208-1-Ig, Clone: C1.18.4), and CoraLite®488-Conjugated AffiniPure Donkey Anti-Mouse IgG(H+L). Cells were then stained with CoraLite® Plus 647 Anti-Human CD11b. Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed. Monocytes were gated.

  • Flow cytometry (FC) experiment of human PBMCs using Anti-Human CD163 (GHI/61) Mouse Recombinant Antibo (65561-1-MR)

    FC experiment of human PBMCs using 65561-1-MR

    1x10^6 human PBMCs were surface stained with 0.5 ug Anti-Human CD163 Mouse Recombinant Antibody (65561-1-MR, Clone: GHI/61) or 0.5 ug Mouse IgG2a Isotype (65208-1-Ig, Clone: C1.18.4) and CoraLite®647-Conjugated Goat Anti-Mouse IgG(H+L). Cells were then stained with FITC Anti-Human CD11b. Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed. Monocytes were gated.

  • Flow cytometry (FC) experiment of human PBMCs using Anti-Human CD163 (GHI/61) Mouse IgG2a Recombinant (65561-1-MR)

    FC experiment of human PBMCs using 65561-1-MR

    1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human CD163 Mouse IgG2a Recombinant Antibody (65561-1-MR, Clone:GHI/61) (red) or Mouse IgG2a Isotype Control (65208-1-Ig, Clone: C1.18.4) (blue), and CoraLite®488-Conjugated AffiniPure Donkey Anti-Mouse IgG(H+L). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed. Monocytes were gated.

  • Flow cytometry (FC) experiment of human PBMCs using Anti-Human CD163 (GHI/61) Mouse IgG2a Recombinant (65561-1-MR)

    FC experiment of human PBMCs using 65561-1-MR

    1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human CD163 Mouse IgG2a Recombinant Antibody (65561-1-MR, Clone:GHI/61) (left) or 0.25 ug Anti-Human CD163 (65169-1-Ig, Clone: GHI/61) (right), and CoraLite®488-Conjugated AffiniPure Donkey Anti-Mouse IgG(H+L). Cells were then stained with CoraLite® Plus 647 Anti-Human CD11b. Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed. Monocytes were gated.

Tested Applications

Positive FC detected inhuman PBMCs

Recommended dilution

ApplicationDilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

65561-1-MR targets CD163 in FC applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG2a
Class Recombinant
Type Antibody
Immunogen Glycoprotein preparation from hairy cell leukemia spleen
Full Name CD163 molecule
Calculated Molecular Weight 1156 aa, 125 kDa
GenBank Accession NumberBC051281
Gene Symbol CD163
Gene ID (NCBI) 9332
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS with 0.09% sodium azide.
Storage ConditionsStore at 2-8°C. Stable for one year after shipment.

Background Information

CD163, also known as M130, is a membrane glycoprotein that belongs to the scavenger receptor superfamily (PMID: 8370408). It is an acute phase-regulated and signal-inducing macrophage protein expressed exclusively in monocytes and tissue macrophages (PMID: 11196644). CD163 mediates endocytosis of haptoglobin-haemoglobin complexes (PMID: 11196644). The uptake of haptoglobin by macrophages contributes to the recycling of iron and also to the inflammatory response (PMID: 22900885). Soluble CD163 (sCD163), as a result of ectodomain shedding during inflammatory activation of macrophages, circulates in blood and has been suggested as a plasma/serum marker for macrophage activity (PMID: 12570164).

Protocols

Product Specific Protocols
FC protocol for CD163 antibody 65561-1-MRDownload protocol
Standard Protocols
Click here to view our Standard Protocols