CoraLite® Plus 750 Anti-Human CD16 (3G8) Mouse IgG2a Recombinant Antibody

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  FC experiment of human peripheral blood leukocytes using CL750-65612

  1x10^6 human peripheral blood leukocytes were surface stained with 5 ul CoraLite® Plus 750 Anti-Human CD16 (3G8) Mouse IgG2a RecAb (CL750-65612, Clone:3G8), or CoraLite® Plus 750 Mouse IgG2a Isotype Control (C1.18.4) (CL750-65208, Clone: C1.18.4). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.

• 

  FC experiment of human peripheral blood leukocytes using CL750-65612

  1x10^6 human peripheral blood leukocytes were surface stained with 5 ul CoraLite® Plus 750 Anti-Human CD16 (3G8) Mouse IgG2a RecAb (CL750-65612, Clone:3G8) (red), or CoraLite® Plus 750 Mouse IgG2a Isotype Control (C1.18.4) (CL750-65208, Clone: C1.18.4)(blue). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.

CD16 Uni-rAb^TM Recombinant Antibody for FC

Cat No. CL750-65612

Clone No.3G8

Host / Isotype

Mouse / IgG2a

Reactivity

human

Applications

FC

Synonyms

Fc gamma RIII alpha, Fc gamma RIII, CD16-II, CD16a antigen, CD16A

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Tested Applications

• Positive FC detected in: human peripheral blood leukocytes

Recommended dilution

Application	Dilution
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 ul per 10^6 cells in a 100 µl suspension or 5 ul per 100 µl of whole blood.
Sample-dependent, Check data in validation data gallery.

Product Information

CL750-65612 targets CD16 in FC applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG2a
• Class: Recombinant
• Type: Antibody
• Immunogen: N/A
• Full Name: Fc fragment of IgG, low affinity IIIa, receptor (CD16a)
• Calculated Molecular Weight: 254 aa, 29 kDa
• GenBank Accession Number: BC017865
• Gene Symbol: CD16a
• Gene ID (NCBI): 2214
• ENSEMBL Gene ID: ENSG00000203747
• Conjugate: CoraLite® Plus 750 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 755 nm / 780 nm
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.09% sodium azide and 0.5% BSA.
• Storage Conditions: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

CD16 is a 50-70-kDa low affinity Fc receptor found on the surface of natural killer cells, neutrophil polymorphonuclear leukocytes, monocytes and macrophages. CD16 mediates antibody-dependent cellular cytotoxicity (ADCC) and other antibody-dependent responses, such as phagocytosis. CD16 has been identified as Fc receptors FcγRIIIa (CD16a) and FcγRIIIb (CD16b), encoded by two nearly identical genes, FCGR3A and the FCGR3B. Clone 3G8 recognizes both the CD16a and CD16b (PMID: 7592758).