Biotin Plus Anti-Human CD16 (3G8) Mouse IgG2a Recombinant Antibody

CD16 Uni-rAb^TM Recombinant Antibody for FC

Host / Isotype

Mouse / IgG2a

Reactivity

human

Applications

FC

Conjugate

Biotin Plus

CloneNo.

3G8

Cat no : Biotin-65612

Synonyms

Fc gamma RIII alpha, Fc gamma RIII, CD16-II, CD16a antigen, CD16A

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Validation Data Gallery

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  FC experiment of human PBMCs using Biotin-65612

  1x10^6 human PBMCs were surface stained with 0.25 ug Biotin Plus Anti-Human CD16 (3G8) Mouse IgG2a Recombinant Antibody (Biotin-65612, Clone: 3G8), or unstained. Cells were not fixed. Lymphocytes were gated.

• 

  FC experiment of human PBMCs using Biotin-65612

  1x10^6 human PBMCs were surface stained with 0.25 ug Biotin Plus Anti-Human CD16 (3G8) Mouse IgG2a Recombinant Antibody (Biotin-65612, Clone: 3G8) (red), or unstained (blue). Cells were not fixed. Lymphocytes were gated.

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Tested Applications

• Positive FC detected in: human PBMCs

Recommended dilution

Application	Dilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

Biotin-65612 targets CD16 in FC applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG2a
• Class: Recombinant
• Type: Antibody
• Immunogen: N/A
• Full Name: Fc fragment of IgG, low affinity IIIa, receptor (CD16a)
• Calculated Molecular Weight: 254 aa, 29 kDa
• GenBank Accession Number: BC017865
• Gene Symbol: CD16a
• Gene ID (NCBI): 2214
• Conjugate: Biotin Plus
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.09% sodium azide.
• Storage Conditions: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

CD16 is a 50-70-kDa low affinity Fc receptor found on the surface of natural killer cells, neutrophil polymorphonuclear leukocytes, monocytes and macrophages. CD16 mediates antibody-dependent cellular cytotoxicity (ADCC) and other antibody-dependent responses, such as phagocytosis. CD16 has been identified as Fc receptors FcγRIIIa (CD16a) and FcγRIIIb (CD16b), encoded by two nearly identical genes, FCGR3A and the FCGR3B. Clone 3G8 recognizes both the CD16a and CD16b (PMID: 7592758).