Anti-Human CD141/Thrombomodulin Rabbit Recombinant Antibody, PBS Only

CD141/Thrombomodulin Uni-rAb^TM Recombinant Antibody for FC

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC

Conjugate

Unconjugated

CloneNo.

240365H3

Cat no : 98037-2-PBS

Synonyms

THBD, Thrombomodulin, THRM, Fetomodulin, CD141

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Validation Data Gallery

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  FC experiment of human peripheral blood leukocytes using 98037-2-RR (same clone as 98037-2-PBS)

  1x10^6 human peripheral blood leukocytes were surface stained with 0.25 ug Anti-Human Thrombomodulin Rabbit Recombinant Antibody (98037-2-RR, Clone: 240365H3) or 0.25 ug Control Antibody, and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed. This data was developed using the same antibody clone with 98037-2-PBS in a different storage buffer formulation.

• 

  FC experiment of human peripheral blood leukocytes using 98037-2-RR (same clone as 98037-2-PBS)

  1x10^6 human peripheral blood leukocytes were surface stained with 0.25 ug Anti-Human Thrombomodulin Rabbit Recombinant Antibody (98037-2-RR, Clone: 240365H3) (red) or 0.25 ug Control Antibody (blue), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed. This data was developed using the same antibody clone with 98037-2-PBS in a different storage buffer formulation.

• 

  Affinity and Kinetic Characterization of 98037-2-RR

  Biolayer interferometry (BLl) kinetic assays of 98037-2-RR against Human CD141/Thrombomodulin were performed. The affinity constant is 0.684 nM.

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Product Information

The immunogen of 98037-2-PBS is CD141/Thrombomodulin Fusion Protein expressed in E. coli.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Fusion Protein
• Full Name: thrombomodulin
• Calculated Molecular Weight: 60 kDa
• GenBank Accession Number: NM_000361.3
• Gene Symbol: THBD
• Gene ID (NCBI): 7056
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

Thrombomodulin, also known as CD141, is an endothelial cell surface glycoprotein that forms a 1:1 complex with the coagulation factor thrombin and plays an important role as a natural anticoagulant. Thrombomodulin serves to convert thrombin from a procoagulant protein into the activator for protein C. Once converted to activated protein C (APC), this protein serves as a major anticoagulant in blood (PMID: 2827310). Thrombomodulin is also located in other cells (keratinocytes, osteoblasts, macrophages,...) where it might be involved in cell differentiation or in inflammation (PMID: 9814688). In humans, thrombomodulin is encoded by the THBD gene. Mutations in this gene are a cause of thromboembolic disease, also known as inherited thrombophilia. Thrombomodulin is glycosylated and has an apparent molecular weight of 75 to 110 kDa (PMID: 1650405; 2827310).