Anti-Human CD141/Thrombomodulin Rabbit Recombinant Antibody

CD141/Thrombomodulin Uni-rAb^TM Recombinant Antibody for FC

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC

Conjugate

Unconjugated

CloneNo.

240365H3

Cat no : 98037-2-RR

Synonyms

THBD, Thrombomodulin, THRM, Fetomodulin, CD141

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Validation Data Gallery

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  FC experiment of human peripheral blood leukocytes using 98037-2-RR

  1x10^6 human peripheral blood leukocytes were surface stained with 0.25 ug Anti-Human Thrombomodulin Rabbit Recombinant Antibody (98037-2-RR, Clone: 240365H3) or 0.25 ug Control Antibody, and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.

• 

  FC experiment of human peripheral blood leukocytes using 98037-2-RR

  1x10^6 human peripheral blood leukocytes were surface stained with 0.25 ug Anti-Human Thrombomodulin Rabbit Recombinant Antibody (98037-2-RR, Clone: 240365H3) (red) or 0.25 ug Control Antibody (blue), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.

• 

  Affinity and Kinetic Characterization of 98037-2-RR

  Biolayer interferometry (BLl) kinetic assays of 98037-2-RR against Human CD141/Thrombomodulin were performed. The affinity constant is 0.684 nM.

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Tested Applications

• Positive FC detected in: human peripheral blood leukocytes

Recommended dilution

Application	Dilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 98037-2-RR is CD141/Thrombomodulin Fusion Protein expressed in E. coli.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Fusion Protein
• Full Name: thrombomodulin
• Calculated Molecular Weight: 60 kDa
• GenBank Accession Number: NM_000361.3
• Gene Symbol: THBD
• Gene ID (NCBI): 7056
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• Storage Buffer: PBS with 0.09% sodium azide, pH 7.3.
• Storage Conditions: Store at 2 - 8°C. Stable for one year after shipment.

Background Information

Thrombomodulin, also known as CD141, is an endothelial cell surface glycoprotein that forms a 1:1 complex with the coagulation factor thrombin and plays an important role as a natural anticoagulant. Thrombomodulin serves to convert thrombin from a procoagulant protein into the activator for protein C. Once converted to activated protein C (APC), this protein serves as a major anticoagulant in blood (PMID: 2827310). Thrombomodulin is also located in other cells (keratinocytes, osteoblasts, macrophages,...) where it might be involved in cell differentiation or in inflammation (PMID: 9814688). In humans, thrombomodulin is encoded by the THBD gene. Mutations in this gene are a cause of thromboembolic disease, also known as inherited thrombophilia. Thrombomodulin is glycosylated and has an apparent molecular weight of 75 to 110 kDa (PMID: 1650405; 2827310).