Anti-Mouse CD14 Rabbit Recombinant Antibody

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  IF Staining of RAW 264.7 using 98020-1-RR

  Immunofluorescent analysis of (4% PFA) fixed RAW 264.7 cells using CD14 antibody (98020-1-RR, Clone: 240225C2 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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  FC experiment of mouse peritoneal macrophages using 98020-1-RR

  1x10^6 mouse peritoneal macrophages were surface stained with 0.25 ug Anti-Mouse CD14 Rabbit Recombinant Antibody (98020-1-RR, Clone: 240225C2) and PE-conjugated Goat Anti-Rabbit IgG (red), or 0.25 ug Isotype Control and PE-conjugated Goat Anti-Rabbit IgG (blue). Cells were not fixed.

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  Affinity and Kinetic Characterization of 98020-1-RR

  Biolayer interferometry (BLl) kinetic assays of 98020-1-RR against Mouse CD14 were performed. The affinity constant is 0.383 nM.

CD14 Uni-rAb^TM Recombinant Antibody for IF/ICC, FC

Cat No. 98020-1-RR

Clone No.240225C2

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

IF/ICC, FC

Synonyms

CD14 antigen, 240225C2

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Tested Applications

• Positive IF/ICC detected in: RAW 264.7 cells
• Positive FC detected in: mouse peritoneal macrophages

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:125-1:500

This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

98020-1-RR targets CD14 in IF/ICC, FC applications and shows reactivity with mouse samples.

• Tested Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Fusion Protein
• Full Name: CD14 antigen
• Calculated Molecular Weight: 39 kDa
• GenBank Accession Number: NM_009841.4
• Gene Symbol: Cd14
• Gene ID (NCBI): 12475
• RRID: AB_3672164
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: P10810
• Storage Buffer: PBS with 0.09% sodium azide.
• Storage Conditions: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

CD14 is a 50-55 kDa glycosylphosphatidylinositol-anchored glycoprotein preferentially expressed on monocytes and macrophages, and at lower levels on granulocytes (PMID: 3385210; 2462937; 7685797). CD14 can also exist as a soluble protein. CD14 acts as a co-receptor for bacterial liposaccharides (LPS) (PMID: 1698311). It plays a major role in the inflammatory response of monocytes to LPS.