Anti-Mouse CD14 Rabbit Recombinant Antibody, PBS Only

CD14 Uni-rAb^TM Recombinant Antibody for IF/ICC, FC

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

IF/ICC, FC

Conjugate

Unconjugated

CloneNo.

240225C2

Cat no : 98020-1-PBS

Synonyms

CD14 antigen, 240225C2

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Validation Data Gallery

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  IF Staining of RAW 264.7 using 98020-1-RR (same clone as 98020-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed RAW 264.7 cells using CD14 antibody (98020-1-RR, Clone: 240225C2 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 98020-1-PBS in a different storage buffer formulation.

• 

  FC experiment of mouse peritoneal macrophages using 98020-1-RR (same clone as 98020-1-PBS)

  1x10^6 mouse peritoneal macrophages were surface stained with 0.25 ug Anti-Mouse CD14 Rabbit Recombinant Antibody (98020-1-RR, Clone: 240225C2) and PE-conjugated Goat Anti-Rabbit IgG (red), or 0.25 ug Isotype Control and PE-conjugated Goat Anti-Rabbit IgG (blue). Cells were not fixed. This data was developed using the same antibody clone with 98020-1-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 98020-1-RR

  Biolayer interferometry (BLl) kinetic assays of 98020-1-RR against Mouse CD14 were performed. The affinity constant is 0.383 nM.

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Product Information

The immunogen of 98020-1-PBS is CD14 Fusion Protein expressed in E. coli.

• Tested Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Fusion Protein
• Full Name: CD14 antigen
• Calculated Molecular Weight: 39 kDa
• GenBank Accession Number: NM_009841.4
• Gene Symbol: Cd14
• Gene ID (NCBI): 12475
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

CD14 is a 50-55 kDa glycosylphosphatidylinositol-anchored glycoprotein preferentially expressed on monocytes and macrophages, and at lower levels on granulocytes (PMID: 3385210; 2462937; 7685797). CD14 can also exist as a soluble protein. CD14 acts as a co-receptor for bacterial liposaccharides (LPS) (PMID: 1698311). It plays a major role in the inflammatory response of monocytes to LPS.