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Anti-Mouse CD14 Rabbit Recombinant Antibody

CD14 Uni-rAbTM Recombinant Antibody for IF/ICC, FC

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

IF/ICC, FC

Conjugate

Unconjugated

CloneNo.

240225C2

Cat no : 98020-1-RR

Synonyms

CD14 antigen, 240225C2

Validation Data Gallery

Filter:
  • Immunofluorescence (IF) / fluorescent staining of RAW 264.7 cells using Anti-Mouse CD14 Rabbit Recombinant Antibody (98020-1-RR)

    IF Staining of RAW 264.7 using 98020-1-RR

    Immunofluorescent analysis of (4% PFA) fixed RAW 264.7 cells using CD14 antibody (98020-1-RR, Clone: 240225C2 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Flow cytometry (FC) experiment of mouse peritoneal macrophages using Anti-Mouse CD14 Rabbit Recombinant Antibody (98020-1-RR)

    FC experiment of mouse peritoneal macrophages using 98020-1-RR

    1x10^6 mouse peritoneal macrophages were surface stained with 0.25 ug Anti-Mouse CD14 Rabbit Recombinant Antibody (98020-1-RR, Clone: 240225C2) and PE-conjugated Goat Anti-Rabbit IgG (red), or 0.25 ug Isotype Control and PE-conjugated Goat Anti-Rabbit IgG (blue). Cells were not fixed.

  • Affinity and Kinetic Characterization of 98020-1-RR

    Affinity and Kinetic Characterization of 98020-1-RR

    Biolayer interferometry (BLl) kinetic assays of 98020-1-RR against Mouse CD14 were performed. The affinity constant is 0.383 nM.

Tested Applications

Positive IF/ICC detected inRAW 264.7 cells
Positive FC detected inmouse peritoneal macrophages

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:125-1:500
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 98020-1-RR is CD14 Fusion Protein expressed in E. coli.

Tested Reactivity mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Fusion Protein
Full Name CD14 antigen
Calculated Molecular Weight39 kDa
GenBank Accession NumberNM_009841.4
Gene Symbol Cd14
Gene ID (NCBI) 12475
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS with 0.09% sodium azide.
Storage ConditionsStore at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

CD14 is a 50-55 kDa glycosylphosphatidylinositol-anchored glycoprotein preferentially expressed on monocytes and macrophages, and at lower levels on granulocytes (PMID: 3385210; 2462937; 7685797). CD14 can also exist as a soluble protein. CD14 acts as a co-receptor for bacterial liposaccharides (LPS) (PMID: 1698311). It plays a major role in the inflammatory response of monocytes to LPS.

Protocols

Product Specific Protocols
IF protocol for CD14 antibody 98020-1-RRDownload protocol
FC protocol for CD14 antibody 98020-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols