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Anti-Human CD126/IL-6R alpha Rabbit Recombinant Antibody, PBS Only

CD126/IL-6R alpha Uni-rAbTM Recombinant Antibody for FC

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC

Conjugate

Unconjugated

CloneNo.

240734A7

Cat no : 98057-1-PBS

Synonyms

CD126, IL-6R alpha, 240734A7, IL 6R alpha, IL 6R 1

Validation Data Gallery

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  • Flow cytometry (FC) experiment of human PBMCs using Anti-Human CD126/IL-6R alpha Rabbit Recombinant An (98057-1-RR)

    FC experiment of human PBMCs using 98057-1-RR (same clone as 98057-1-PBS)

    1x10^6 human PBMCs were stained with CoraLite® Plus 647 Anti-Human CD3 (OKT3) Mouse IgG2a Recombinant Antibody (CL647-65569, Clone: OKT3) and 0.25 ug Anti-Human CD126/IL-6R alpha Rabbit Recombinant Antibody (98057-1-RR, Clone: 240734A7), or Isotype Control. Cells were not fixed. This data was developed using the same antibody clone with 98057-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of human PBMCs using Anti-Human CD126/IL-6R alpha Rabbit Recombinant An (98057-1-RR)

    FC experiment of human PBMCs using 98057-1-RR (same clone as 98057-1-PBS)

    1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human CD126/IL-6R alpha Rabbit Recombinant Antibody (98057-1-RR, Clone:240734A7) (red), or Isotype Control (blue). Cells were not fixed. This data was developed using the same antibody clone with 98057-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 98057-1-RR

    Affinity and Kinetic Characterization of 98057-1-RR

    Biolayer interferometry (BLl) kinetic assays of 98057-1-RR against Human CD126/IL-6R alpha were performed. The affinity constant is below 1 pM.

Product Information

The immunogen of 98057-1-PBS is CD126/IL-6R alpha Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Fusion Protein
Full Name interleukin 6 receptor
Calculated Molecular Weight 468 aa, 52 kDa
GenBank Accession NumberBC132684
Gene Symbol IL-6R alpha
Gene ID (NCBI) 3570
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Interleukin-6 (IL-6) is a pleiotropic cytokine produced by a variety of cells during infection, trauma, and immunological challenge (PMID: 8274730). IL-6 acts via a receptor complex consisting of two distinct membrane-bound glycoproteins, an 80-kDa IL-6-binding subunit (IL-6R alpha, CD126, gp80) and a 130-kDa signal-transducing element (gp130, CD130). After binding of IL-6 to membrane-bound IL-6R alpha, the complex of IL-6 and IL-6R alpha associates with gp130, thus activating the receptor (PMID: 9716487). Expression of gp130 is found in almost all organs while expression of IL-6R alpha is predominantly confined to hepatocytes and leukocyte subpopulations (monocytes, neutrophils, T cells, and B cells) (PMID: 11149892). Soluble form of IL-6R alpha has been found in human serum and urine (PMID: 2529343; 1545125).