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Anti-Human CD126/IL-6R alpha Rabbit Recombinant Antibody

CD126/IL-6R alpha Uni-rAbTM Recombinant Antibody for FC

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC

Conjugate

Unconjugated

CloneNo.

240734A7

Cat no : 98057-1-RR

Synonyms

CD126, IL-6R alpha, 240734A7, IL 6R alpha, IL 6R 1

Validation Data Gallery

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  • Flow cytometry (FC) experiment of human PBMCs using Anti-Human CD126/IL-6R alpha Rabbit Recombinant An (98057-1-RR)

    FC experiment of human PBMCs using 98057-1-RR

    1x10^6 human PBMCs were stained with CoraLite® Plus 647 Anti-Human CD3 (OKT3) Mouse IgG2a Recombinant Antibody (CL647-65569, Clone: OKT3) and 0.25 ug Anti-Human CD126/IL-6R alpha Rabbit Recombinant Antibody (98057-1-RR, Clone: 240734A7), or Isotype Control. Cells were not fixed.

  • Flow cytometry (FC) experiment of human PBMCs using Anti-Human CD126/IL-6R alpha Rabbit Recombinant An (98057-1-RR)

    FC experiment of human PBMCs using 98057-1-RR

    1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human CD126/IL-6R alpha Rabbit Recombinant Antibody (98057-1-RR, Clone:240734A7) (red), or Isotype Control (blue). Cells were not fixed.

  • Affinity and Kinetic Characterization of 98057-1-RR

    Affinity and Kinetic Characterization of 98057-1-RR

    Biolayer interferometry (BLl) kinetic assays of 98057-1-RR against Human CD126/IL-6R alpha were performed. The affinity constant is below 1 pM.

Tested Applications

Positive FC detected inhuman PBMCs

Recommended dilution

ApplicationDilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 98057-1-RR is CD126/IL-6R alpha Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Fusion Protein
Full Name interleukin 6 receptor
Calculated Molecular Weight 468 aa, 52 kDa
GenBank Accession NumberBC132684
Gene Symbol IL-6R alpha
Gene ID (NCBI) 3570
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS with 0.09% sodium azide, pH 7.3.
Storage ConditionsStore at 2-8°C. Stable for one year after shipment.

Background Information

Interleukin-6 (IL-6) is a pleiotropic cytokine produced by a variety of cells during infection, trauma, and immunological challenge (PMID: 8274730). IL-6 acts via a receptor complex consisting of two distinct membrane-bound glycoproteins, an 80-kDa IL-6-binding subunit (IL-6R alpha, CD126, gp80) and a 130-kDa signal-transducing element (gp130, CD130). After binding of IL-6 to membrane-bound IL-6R alpha, the complex of IL-6 and IL-6R alpha associates with gp130, thus activating the receptor (PMID: 9716487). Expression of gp130 is found in almost all organs while expression of IL-6R alpha is predominantly confined to hepatocytes and leukocyte subpopulations (monocytes, neutrophils, T cells, and B cells) (PMID: 11149892). Soluble form of IL-6R alpha has been found in human serum and urine (PMID: 2529343; 1545125).

Protocols

Product Specific Protocols
FC protocol for CD126/IL-6R alpha antibody 98057-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols