CoraLite® Plus 647 Anti-Rat CD11b (OX42)

CD11b Monoclonal Antibody for FC

Host / Isotype

Mouse / IgG2a, kappa

Reactivity

Rat

Applications

FC

Conjugate

CoraLite® Plus 647 Fluorescent Dye

CloneNo.

OX42

Cat no : CL647-65229

Synonyms

Cd11b, integrin alpha M, Itgam

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Validation Data Gallery

• 

  FC experiment of wistar rat splenocytes using CL647-65229

  1X10^6 Wistar rat splenocytes were surface stained with 0.5 ug CoraLite® Plus 647 Anti-Rat CD11b (CL647-65229, Clone: OX42) or 5 ul Control Antibody. Cells were not fixed.

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Tested Applications

• Positive FC detected in: Wistar rat splenocytes

Recommended dilution

Application	Dilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL647-65229 targets CD11b in FC applications and shows reactivity with Rat samples.

• Tested Reactivity: Rat
• Host / Isotype: Mouse / IgG2a, kappa
• Class: Monoclonal
• Type: Antibody
• Immunogen: Rat peritoneal macrophages
• Full Name: integrin alpha M
• Calculated Molecular Weight: 127 kDa
• GenBank Accession Number: NM_012711
• Gene Symbol: CD11b
• Gene ID (NCBI): 25021
• Conjugate: CoraLite® Plus 647 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 654 nm / 674 nm
• Form: Liquid
• Purification Method: Affinity purification
• Storage Buffer: PBS with 0.09% sodium azide.
• Storage Conditions: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

Integrins are cell adhesion receptors that are heterodimers composed of non-covalently associated α and β subunits (PMID: 9779984). CD11b, also known as Integrin alpha M or CR3A, belongs to the integrin alpha chain family. CD11b forms an α/β heterodimer with CD18 (integrin β2). CD11b/CD18 is implicated in various adhesive interactions of monocytes, macrophages and granulocytes as well as in mediating the uptake of complement-coated particles and pathogens (PMID: 9558116; 20008295). CD11b/CD18 is a receptor for the complement protein fragment iC3b, and is also a receptor for fibrinogen, factor X and ICAM1 (PMID: 2971974; 15485828).