Anti-Rat CD11b (OX42)

CD11b Monoclonal Antibody for FC

Host / Isotype

Mouse / IgG2a, kappa

Reactivity

rat and More (1)

Applications

FC

Conjugate

Unconjugated

Publications

2

CloneNo.

OX42

Cat no : 65229-1-Ig

Synonyms

OX42, Itgam, integrin alpha M

---

Validation Data Gallery

• 

  FC experiment of LOU rat splenocytes using 65229-1-Ig

  1X10^6 LOU rat splenocytes were surface stained with 0.5 ug Anti-Rat CD11b (65229-1-Ig, Clone: OX42) and FITC anti-Mouse IgG2a Antibody at dilution 1:100, or 0.5 ug Control Antibody. Cells were not fixed.

---

Tested Applications

• Positive FC detected in: LOU rat splenocytes

Recommended dilution

Application	Dilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

65229-1-Ig targets CD11b in FC applications and shows reactivity with rat samples.

• Tested Reactivity: rat
• Cited Reactivity: mouse
• Host / Isotype: Mouse / IgG2a, kappa
• Class: Monoclonal
• Type: Antibody
• Immunogen: Rat peritoneal macrophages
• Full Name: integrin alpha M
• Calculated Molecular Weight: 127 kDa
• GenBank Accession Number: NM_012711
• Gene Symbol: CD11b
• Gene ID (NCBI): 25021
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at 2-8°C. Stable for one year after shipment.

Background Information

Integrins are cell adhesion receptors that are heterodimers composed of non-covalently associated α and β subunits (PMID: 9779984). CD11b, also known as Integrin alpha M or CR3A, belongs to the integrin alpha chain family. CD11b forms an α/β heterodimer with CD18 (integrin β2). CD11b/CD18 is implicated in various adhesive interactions of monocytes, macrophages and granulocytes as well as in mediating the uptake of complement-coated particles and pathogens (PMID: 9558116; 20008295). CD11b/CD18 is a receptor for the complement protein fragment iC3b, and is also a receptor for fibrinogen, factor X and ICAM1 (PMID: 2971974; 15485828).

Publications

Species	Application	Title
mouse	WB	Food Funct; Orally administered neohesperidin attenuates MPTP-induced neurodegeneration by inhibiting inflammatory responses and regulating intestinal flora in mice; Authors - Dewei He
mouse		J Agric Food Chem; Orally Administered Ginkgolide C Alleviates MPTP-Induced Neurodegeneration by Suppressing Neuroinflammation and Oxidative Stress through Microbiota-Gut-Brain Axis in Mice; Authors - Xiyu Gao