CoraLite® Plus 405 Anti-Mouse CD11a (M17/4)

• 

  FC experiment of C57BL/6 mouse thymocytes using CL405-65177

  1X10^6 C57BL/6 mouse thymocytes were surface stained with 0.5 ug CoraLite® Plus 405 Anti-Mouse CD11a (CL405-65177, Clone:M17/4), or 0.5 ug CoraLite® Plus 405 Rat IgG2a Isotype Control (2A3) (CL405-65209, Clone: 2A3). Cells were not fixed.

• 

  FC experiment of mouse splenocytes using CL405-65177

  1X10^6 mouse splenocytes were surface stained with 0.5 ug CoraLite® Plus 405 Anti-Mouse CD11a (CL405-65177, Clone: M17/4) (red), or 0.5 ug Control Antibody. Cells were not fixed.

CD11a Monoclonal Antibody for FC

Cat No. CL405-65177

Clone No.M17/4

Host / Isotype

Rat / IgG2a, kappa

Reactivity

Mouse

Applications

FC

Synonyms

Cd11a, integrin alpha L, Itgal, LFA 1, Ly 15, Ly 21

---

Tested Applications

• Positive FC detected in: mouse thymocytes, mouse splenocytes

Recommended dilution

Application	Dilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL405-65177 targets CD11a in FC applications and shows reactivity with Mouse samples.

• Tested Reactivity: Mouse
• Host / Isotype: Rat / IgG2a, kappa
• Class: Monoclonal
• Type: Antibody
• Immunogen: C57BL/6 mouse splenic secondary cytotoxic T cells
• Full Name: integrin alpha L
• GenBank Accession Number: BC145802
• Gene Symbol: CD11a
• Gene ID (NCBI): 16408
• RRID: AB_2934367
• Conjugate: CoraLite® Plus 405 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 399 nm / 422 nm
• Form: Liquid
• Purification Method: Purified by protein-A affinity chromatography
• UNIPROT ID: B7ZN92
• Storage Buffer: PBS with 0.09% sodium azide.
• Storage Conditions: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

CD11a, also known as integrin αL or LFA-1α, is a 170-180 kDa transmembrane glycoprotein that forms a heterodimer with CD18 (PMID: 7027264; 1672643). CD11a/CD18 (LFA-1) is expressed by all leukocytes and mediates cell adhesion through interactions with its ligands, intercellular adhesion molecule 1 (ICAM-1), ICAM-2, and ICAM-3 (PMID: 7479767). CD11a/CD18 also functions in lymphocyte costimulatory signaling (PMID: 1972160).