CD11b Monoclonal antibody, PBS Only

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  WB analysis of TF-1 using 66519-1-Ig (same clone as 66519-1-PBS)

  TF-1 cells were subjected to SDS PAGE followed by western blot with 66519-1-Ig (CD11B/Integrin alpha M antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66519-1-PBS in a different storage buffer formulation.

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  WB analysis of human bone marrow using 66519-1-Ig (same clone as 66519-1-PBS)

  human bone marrow tissue were subjected to SDS PAGE followed by western blot with 66519-1-Ig (CD11B/Integrin alpha M antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66519-1-PBS in a different storage buffer formulation.

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  IHC staining of human tonsillitis using 66519-1-Ig (same clone as 66519-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66519-1-Ig (CD11B/Integrin alpha M antibody) at dilution of 1:1000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66519-1-PBS in a different storage buffer formulation.

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  IHC staining of human tonsillitis using 66519-1-Ig (same clone as 66519-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66519-1-Ig (CD11B/Integrin alpha M antibody) at dilution of 1:1000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66519-1-PBS in a different storage buffer formulation.

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  IF Staining of human tonsillitis using 66519-1-Ig (same clone as 66519-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using 66519-1-Ig (CD11B/Integrin alpha M antibody) at dilution of 1:100 and CoraLite488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66519-1-PBS in a different storage buffer formulation.

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  IF Staining of human tonsillitis using 66519-1-Ig (same clone as 66519-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human tonsillitis tissue using CD11B/Integrin Alpha M antibody (66519-1-Ig, Clone: 1C7C2 ) at dilution of 1:200 and CoraLite®594-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-3). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66519-1-PBS in a different storage buffer formulation.

CD11b Monoclonal Antibody for WB, IHC, IF-P, ELISA

Cat No. 66519-1-PBS

Clone No.1C7C2

Host / Isotype

Mouse / IgG2b

Reactivity

human

Applications

WB, IHC, IF-P, ELISA

Synonyms

1C7C2, CD11 antigen-like family member B, Cell surface glycoprotein MAC-1 subunit alpha, CR 3 alpha chain, CR-3 alpha chain

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Product Information

66519-1-PBS targets CD11b in WB, IHC, IF-P, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  Peptide

• Full Name: integrin, alpha M (complement component 3 receptor 3 subunit)
• Calculated Molecular Weight: 93 kDa
• Observed Molecular Weight: 160 kDa
• GenBank Accession Number: NM_000632
• Gene Symbol: CD11b
• Gene ID (NCBI): 3684
• ENSEMBL Gene ID: ENSG00000169896
• RRID: AB_2881882
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P11215
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

Integrins are cell adhesion receptors that are heterodimers composed of non-covalently associated α and β subunits (PMID: 9779984). CD11b, also known as Integrin alpha M or CR3A, belongs to the integrin alpha chain family. CD11b forms an α/β heterodimer with CD18 (integrin β2). CD11b/CD18 is implicated in various adhesive interactions of monocytes, macrophages and granulocytes as well as in mediating the uptake of complement-coated particles and pathogens (PMID: 9558116; 20008295). CD11b/CD18 is a receptor for the complement protein fragment iC3b, and is also a receptor for fibrinogen, factor X and ICAM1 (PMID: 2971974; 15485828).