CD11B/Integrin Alpha M Monoclonal antibody

CD11B/Integrin Alpha M Monoclonal Antibody for WB, IHC, IF-P, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Human and More (1)

Applications

WB, IHC, IF-P, ELISA

Conjugate

Unconjugated

Publications

43

CloneNo.

1C7C2

Reviews (2)

Cat no : 66519-1-Ig

Synonyms

CD11B, CD11B/Integrin Alpha M, CR 3 alpha chain, CR3A, Integrin alpha M, ITGAM, MAC 1, MAC1A, MO1A, Neutrophil adherence receptor, SLEB6

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Validation Data Gallery

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  WB analysis of THP-1 using 66519-1-Ig

  THP-1 cells were subjected to SDS PAGE followed by western blot with 66519-1-Ig (CD11B/Integrin alpha M antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human bone marrow using 66519-1-Ig

  human bone marrow tissue were subjected to SDS PAGE followed by western blot with 66519-1-Ig (CD11B/Integrin alpha M antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of TF-1 using 66519-1-Ig

  TF-1 cells were subjected to SDS PAGE followed by western blot with 66519-1-Ig (CD11B/Integrin alpha M antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human tonsillitis using 66519-1-Ig

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66519-1-Ig (CD11B/Integrin alpha M antibody) at dilution of 1:1000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsillitis using 66519-1-Ig

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66519-1-Ig (CD11B/Integrin alpha M antibody) at dilution of 1:1000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of human tonsillitis using 66519-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using 66519-1-Ig (CD11B/Integrin alpha M antibody) at dilution of 1:100 and CoraLite488-Conjugated Goat Anti-Mouse IgG(H+L).

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Tested Applications

• Positive WB detected in: THP-1 cells, TF-1 cells, human bone marrow tissue
• Positive IHC detected in: human tonsillitis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF-P detected in: human tonsillitis tissue

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochemistry (IHC): IHC : 1:500-1:2000
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 15 publications below
• IHC: See 5 publications below
• IF: See 24 publications below
• FC: See 1 publications below

Product Information

66519-1-Ig targets CD11B/Integrin Alpha M in WB, IHC, IF-P, ELISA applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Cited Reactivity: human
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: integrin, alpha M (complement component 3 receptor 3 subunit)
• Calculated Molecular Weight: 93 kDa
• Observed Molecular Weight: 160 kDa
• GenBank Accession Number: NM_000632
• Gene Symbol: CD11b
• Gene ID (NCBI): 3684
• RRID: AB_2881882
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Integrins are cell adhesion receptors that are heterodimers composed of non-covalently associated α and β subunits (PMID: 9779984). CD11b, also known as Integrin alpha M or CR3A, belongs to the integrin alpha chain family. CD11b forms an α/β heterodimer with CD18 (integrin β2). CD11b/CD18 is implicated in various adhesive interactions of monocytes, macrophages and granulocytes as well as in mediating the uptake of complement-coated particles and pathogens (PMID: 9558116; 20008295). CD11b/CD18 is a receptor for the complement protein fragment iC3b, and is also a receptor for fibrinogen, factor X and ICAM1 (PMID: 2971974; 15485828).

Publications

Species	Application	Title
	IF	J Exp Med; TIPE2 specifies the functional polarization of myeloid-derived suppressor cells during tumorigenesis.; Authors - Dehong Yan
human	IF	Cell Rep; Inflammatory dendritic cells restrain CD11b+CD4+ CTLs via CD200R in human NSCLC; Authors - Mingjie Lin
	FC	Bioact Mater; Photosynthetic microorganisms coupled photodynamic therapy for enhanced antitumor immune effect.; Authors - Haoran Wang
	IF	Apoptosis; ITGAM-macrophage modulation as a potential strategy for treating neutrophilic Asthma: insights from bioinformatics analysis and in vivo experiments; Authors - Qian Yan
	WB	J Neuroinflammation; C1q/TNF-related protein 4 restores leptin sensitivity by downregulating NF-κB signaling and microglial activation.; Authors - Liu Ye
human	WB	Int Immunopharmacol; Restoration of miR-299-3p promotes macrophage phagocytosis and suppresses malignant phenotypes in breast cancer carcinogenesis via dual-targeting CD47 and ABCE1; Authors - Shoufang Tong

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Reyes (Verified Customer) (09-05-2024)

CD11b did not work as good as expected, not showing my microglia, but rather appearing in some small neurons, and most of the marking seems to be autofluorescence from the human brain tissue.

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:100
• Cell Tissue Type: Human Brain Cortex

CD11B/Integrin Alpha M Antibody Immunofluorescence validation (1:100 dilution) in Human Brain Cortex (Cat no:66519-1-Ig)

FH

Santhosh (Verified Customer) (04-15-2019)

Human FFPE tonsil tissue, HIER pH6

• Applications: Immunohistochemistry,
• Primary Antibody Dilution: 1:200

CD11B/Integrin Alpha M Antibody Immunohistochemistry, validation (1:200 dilution) in (Cat no:66519-1-Ig)