CD11B/Integrin Alpha M Monoclonal antibody
CD11B/Integrin Alpha M Monoclonal Antibody for WB, IHC, IF-P, ELISA
Host / Isotype
Mouse / IgG2b
Reactivity
Human and More (1)
Applications
WB, IHC, IF-P, ELISA
Conjugate
Unconjugated
CloneNo.
1C7C2
Cat no : 66519-1-Ig
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | THP-1 cells, TF-1 cells, human bone marrow tissue |
Positive IHC detected in | human tonsillitis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF-P detected in | human tonsillitis tissue |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:1000-1:4000 |
Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Published Applications
KD/KO | See 1 publications below |
WB | See 15 publications below |
IHC | See 5 publications below |
IF | See 21 publications below |
FC | See 1 publications below |
Product Information
66519-1-Ig targets CD11B/Integrin Alpha M in WB, IHC, IF-P, ELISA applications and shows reactivity with Human samples.
Tested Reactivity | Human |
Cited Reactivity | human |
Host / Isotype | Mouse / IgG2b |
Class | Monoclonal |
Type | Antibody |
Immunogen | Peptide |
Full Name | integrin, alpha M (complement component 3 receptor 3 subunit) |
Calculated Molecular Weight | 93 kDa |
Observed Molecular Weight | 160 kDa |
GenBank Accession Number | NM_000632 |
Gene Symbol | CD11b |
Gene ID (NCBI) | 3684 |
RRID | AB_2881882 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Integrins are cell adhesion receptors that are heterodimers composed of non-covalently associated α and β subunits (PMID: 9779984). CD11b, also known as Integrin alpha M or CR3A, belongs to the integrin alpha chain family. CD11b forms an α/β heterodimer with CD18 (integrin β2). CD11b/CD18 is implicated in various adhesive interactions of monocytes, macrophages and granulocytes as well as in mediating the uptake of complement-coated particles and pathogens (PMID: 9558116; 20008295). CD11b/CD18 is a receptor for the complement protein fragment iC3b, and is also a receptor for fibrinogen, factor X and ICAM1 (PMID: 2971974; 15485828).
Protocols
Product Specific Protocols | |
---|---|
WB protocol for CD11B/Integrin Alpha M antibody 66519-1-Ig | Download protocol |
IHC protocol for CD11B/Integrin Alpha M antibody 66519-1-Ig | Download protocol |
IF protocol for CD11B/Integrin Alpha M antibody 66519-1-Ig | Download protocol |
FC protocol for CD11B/Integrin Alpha M antibody 66519-1-Ig | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
J Exp Med TIPE2 specifies the functional polarization of myeloid-derived suppressor cells during tumorigenesis. | ||
Bioact Mater Photosynthetic microorganisms coupled photodynamic therapy for enhanced antitumor immune effect. | ||
Apoptosis ITGAM-macrophage modulation as a potential strategy for treating neutrophilic Asthma: insights from bioinformatics analysis and in vivo experiments | ||
J Neuroinflammation C1q/TNF-related protein 4 restores leptin sensitivity by downregulating NF-κB signaling and microglial activation. | ||
Int Immunopharmacol Restoration of miR-299-3p promotes macrophage phagocytosis and suppresses malignant phenotypes in breast cancer carcinogenesis via dual-targeting CD47 and ABCE1 |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
FH Santhosh (Verified Customer) (04-15-2019) | Human FFPE tonsil tissue, HIER pH6
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