CD11B/Integrin Alpha M Monoclonal antibody
CD11B/Integrin Alpha M Monoclonal Antibody for WB, IHC, IF-P, ELISA
Host / Isotype
Mouse / IgG2b
Reactivity
Human and More (1)
Applications
WB, IHC, IF-P, ELISA
Conjugate
Unconjugated
CloneNo.
1C7C2
Cat no : 66519-1-Ig
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | THP-1 cells, TF-1 cells, human bone marrow tissue |
| Positive IHC detected in | human tonsillitis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | human tonsillitis tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 1 publications below |
| WB | See 15 publications below |
| IHC | See 5 publications below |
| IF | See 24 publications below |
| FC | See 1 publications below |
Product Information
66519-1-Ig targets CD11B/Integrin Alpha M in WB, IHC, IF-P, ELISA applications and shows reactivity with Human samples.
| Tested Reactivity | Human |
| Cited Reactivity | human |
| Host / Isotype | Mouse / IgG2b |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | Peptide |
| Full Name | integrin, alpha M (complement component 3 receptor 3 subunit) |
| Calculated Molecular Weight | 93 kDa |
| Observed Molecular Weight | 160 kDa |
| GenBank Accession Number | NM_000632 |
| Gene Symbol | CD11b |
| Gene ID (NCBI) | 3684 |
| RRID | AB_2881882 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Integrins are cell adhesion receptors that are heterodimers composed of non-covalently associated α and β subunits (PMID: 9779984). CD11b, also known as Integrin alpha M or CR3A, belongs to the integrin alpha chain family. CD11b forms an α/β heterodimer with CD18 (integrin β2). CD11b/CD18 is implicated in various adhesive interactions of monocytes, macrophages and granulocytes as well as in mediating the uptake of complement-coated particles and pathogens (PMID: 9558116; 20008295). CD11b/CD18 is a receptor for the complement protein fragment iC3b, and is also a receptor for fibrinogen, factor X and ICAM1 (PMID: 2971974; 15485828).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for CD11B/Integrin Alpha M antibody 66519-1-Ig | Download protocol |
| IHC protocol for CD11B/Integrin Alpha M antibody 66519-1-Ig | Download protocol |
| IF protocol for CD11B/Integrin Alpha M antibody 66519-1-Ig | Download protocol |
| FC protocol for CD11B/Integrin Alpha M antibody 66519-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
J Exp Med TIPE2 specifies the functional polarization of myeloid-derived suppressor cells during tumorigenesis. | ||
Bioact Mater Photosynthetic microorganisms coupled photodynamic therapy for enhanced antitumor immune effect. | ||
Apoptosis ITGAM-macrophage modulation as a potential strategy for treating neutrophilic Asthma: insights from bioinformatics analysis and in vivo experiments | ||
J Neuroinflammation C1q/TNF-related protein 4 restores leptin sensitivity by downregulating NF-κB signaling and microglial activation. | ||
Int Immunopharmacol Restoration of miR-299-3p promotes macrophage phagocytosis and suppresses malignant phenotypes in breast cancer carcinogenesis via dual-targeting CD47 and ABCE1 |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
FH Reyes (Verified Customer) (09-05-2024) | CD11b did not work as good as expected, not showing my microglia, but rather appearing in some small neurons, and most of the marking seems to be autofluorescence from the human brain tissue.
 |
FH Santhosh (Verified Customer) (04-15-2019) | Human FFPE tonsil tissue, HIER pH6
 |
