PE Anti-Human CD117/c-Kit (104D2)

CD117/c-Kit Monoclonal Antibody for FC

Host / Isotype

Mouse / IgG1, kappa

Reactivity

Human, Bovine

Applications

FC

Conjugate

PE Fluorescent Dye

CloneNo.

104D2

Cat no : PE-65154

Synonyms

C Kit, CD117, KIT, PBT, Proto oncogene c Kit, SCFR, Tyrosine protein kinase Kit

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Validation Data Gallery

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  FC experiment of TF-1 using PE-65154

  1X10^6 TF-1 cells were surface stained with 20.00 ul/test PE Anti-Human CD117 (PE-65154, Clone:104D2) (red) or isotype control antibody (blue). Cells were not fixed.

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Tested Applications

• Positive FC detected in: TF-1 cells

Recommended dilution

Application	Dilution
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 20 µl per 10^6 cells in a 100 µl suspension or 20 µl per 100 µl of whole blood.
Sample-dependent, Check data in validation data gallery.

Product Information

PE-65154 targets CD117/c-Kit in FC applications and shows reactivity with Human, Bovine samples.

• Tested Reactivity: Human, Bovine
• Host / Isotype: Mouse / IgG1, kappa
• Class: Monoclonal
• Type: Antibody
• Immunogen: MOLM-1 megakaryocytic cells
• Full Name: v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog
• Calculated Molecular Weight: 976 aa, 110 kDa
• GenBank Accession Number: BC071593
• Gene Symbol: KIT
• Gene ID (NCBI): 3815
• RRID: AB_2883933
• Conjugate: PE Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 496 nm, 565 nm / 578 nm
• Form: Liquid
• Purification Method: The purified antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. The conjugate is purified by size-exclusion chromatography.
• Storage Buffer: PBS with 0.1% sodium azide.
• Storage Conditions: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

CD117, also known as c-Kit and SCFR, is a transmembrane protein with tyrosine kinase activity encoded by the oncogene c-kit (PMID: 2448137). It is a member of the type III receptor tyrosine kinase family, which also includes CSF-1R, PDGFRβ, PDGFRα, and FLT3 (PMID: 29518044). CD117 is expressed on hematopoietic stem cells and progenitor cells, mast cells, and is also found in a wide range of non-haemopoietic cell types (including melanocytes, germ cells, astrocytes, renal tubules, breast glandular epithelial cells, sweat glands, and interstitial cells of Cajal) (PMID: 10582338; 23073628). CD117 plays an important role in early haemopoiesis. It is also involved in pigmentation, fertility, gut movement, and some aspects of the nervous system (PMID: 23073628).