Anti-Human CD117/c-Kit (104D2)

CD117/c-Kit Monoclonal Antibody for FC

Host / Isotype

Mouse / IgG1, kappa

Reactivity

Human, Bovine

Applications

FC

Conjugate

Unconjugated

CloneNo.

104D2

Cat no : 65154-1-Ig

Synonyms

C Kit, CD117, KIT, PBT, Proto oncogene c Kit, SCFR, Tyrosine protein kinase Kit

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Validation Data Gallery

• 

  FC experiment of TF-1 using 65154-1-Ig

  1X10^6 TF-1 cells were surface stained with 1.5 ug Anti-Human CD117 (65154-1-Ig, Clone:104D2) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (green), or stained with 1.5 ug Mouse IgG1 Isotype Control Antibody and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (black). Cells were not fixed.

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Tested Applications

• Positive FC detected in: TF-1 cells

Recommended dilution

Application	Dilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

65154-1-Ig targets CD117/c-Kit in FC applications and shows reactivity with Human, Bovine samples.

• Tested Reactivity: Human, Bovine
• Host / Isotype: Mouse / IgG1, kappa
• Class: Monoclonal
• Type: Antibody
• Immunogen: MOLM-1 megakaryocytic cells
• Full Name: v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog
• Calculated Molecular Weight: 976 aa, 110 kDa
• GenBank Accession Number: BC071593
• Gene Symbol: CD117
• Gene ID (NCBI): 3815
• RRID: AB_2918436
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Purified by protein-A affinity chromatography
• Storage Buffer: PBS with 0.1% sodium azide.
• Storage Conditions: Store at 2-8°C. Stable for one year after shipment.

Background Information

CD117, also known as c-Kit and SCFR, is a transmembrane protein with tyrosine kinase activity encoded by the oncogene c-kit (PMID: 2448137). It is a member of the type III receptor tyrosine kinase family, which also includes CSF-1R, PDGFRβ, PDGFRα, and FLT3 (PMID: 29518044). CD117 is expressed on hematopoietic stem cells and progenitor cells, mast cells, and is also found in a wide range of non-haemopoietic cell types (including melanocytes, germ cells, astrocytes, renal tubules, breast glandular epithelial cells, sweat glands, and interstitial cells of Cajal) (PMID: 10582338; 23073628). CD117 plays an important role in early haemopoiesis. It is also involved in pigmentation, fertility, gut movement, and some aspects of the nervous system (PMID: 23073628).