CoraLite® Plus 488 Anti-Mouse CD107b / LAMP2 (ABL-93)

CD107b / LAMP2 Monoclonal Antibody for IF/ICC, FC (Intra)

Host / Isotype

Rat / IgG2a, kappa

Reactivity

Mouse

Applications

IF/ICC, FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

ABL-93

Cat no : CL488-65052

Synonyms

CD107b, Lamp 2, Lamp 2a, Lamp 2b, Lamp 2c, Lamp2, Mac3

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Validation Data Gallery

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  IF Staining of NIH/3T3 using CL488-65052

  Immunofluorescent analysis of (-20°C Ethanol) fixed NIH/3T3 cells using CoraLite® Plus 488 CD107b antibody (CL488-65052, Clone: ABL-93 ) at dilution of 1:400.

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  FC experiment of NIH/3T3 using CL488-65052

  1X10^6 NIH/3T3 cells were intracellularly stained with 0.5 ug CoraLite® Plus 488 Anti-Mouse CD107b / LAMP2 (CL488-65052, Clone:ABL-93) (red), or 0.5 ug CoraLite® Plus 488 Rat IgG2a Isotype Control (2A3) (CL488-65209, Clone: 2A3) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

• Positive IF/ICC detected in: NIH/3T3 cells
• Positive FC (Intra) detected in: NIH/3T3 cells
• Positive FC detected in: NIH/3T3 cells

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL488-65052 targets CD107b / LAMP2 in IF/ICC, FC (Intra) applications and shows reactivity with Mouse samples.

• Tested Reactivity: Mouse
• Host / Isotype: Rat / IgG2a, kappa
• Class: Monoclonal
• Type: Antibody
• Immunogen: Glycoproteins purified from BALB/c mouse embryo 3T3 cell line
• Full Name: lysosomal-associated membrane protein 2
• GenBank Accession Number: BC138718
• Gene Symbol: CD107b
• Gene ID (NCBI): 16784
• RRID: AB_2883150
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Affinity purification
• Storage Buffer: PBS with 0.1% sodium azide and 0.5% BSA, pH 7.3.
• Storage Conditions: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

LAMP2 (CD107b) is a Lysosomal membrane glycoprotein. LAMP2 is extensively glycosylated with asparagine-linked oligosaccharides which protect it from intracellular proteolysis (PMID: 10521503). Although LAMP-2 is localized primarily in the endosome-lysosomal membrane of cells, it is also found on the plasma membrane under certain circumstances, e.g., after platelet activation, during granulocytic differentiation and activation, and in some tumor cells (PMID: 12221139). LAMP is involved in lysosomal stability and autophagy (PMID: 12221139). This glycoprotein provides selectins with carbohydrate ligands. LAMP2 may plays a role in tumor cell metastasis (PMID 9426697).