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CoraLite® Plus 647 Anti-Mouse CD107b / LAMP2 (ABL-93)

CD107b / LAMP2 Monoclonal Antibody for IF, FC (Intra)

Host / Isotype

Rat / IgG2a, kappa

Reactivity

Mouse

Applications

IF, FC (Intra)

Conjugate

CoraLite® Plus 647 Fluorescent Dye

CloneNo.

ABL-93

Cat no : CL647-65052

Synonyms

CD107b, Lamp 2, Lamp 2a, Lamp 2b, Lamp 2c, Lamp2, Mac3

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of NIH/3T3 cells using CoraLite® Plus 647 Anti-Mouse CD107b / LAMP2 (ABL- (CL647-65052)

    IF Staining of NIH/3T3 using CL647-65052

    Immunofluorescent analysis of (-20°C Ethanol) fixed NIH/3T3 cells using CoraLite® Plus 647 CD107b antibody (CL647-65052, Clone: ABL-93 ) at dilution of 1:400.

  • Flow cytometry (FC) experiment of NIH/3T3 cells using CoraLite® Plus 647 Anti-Mouse CD107b / LAMP2 (ABL- (CL647-65052)

    FC experiment of NIH/3T3 using CL647-65052

    1x10^6 NIH/3T3 cells were intracellularly stained with 0.25 ug CoraLite® Plus 647 Anti-Mouse CD107b / LAMP2 (CL647-65052, Clone:ABL-93) (red), or 0.25 ug CoraLite® Plus 647 Rat IgG2a Isotype Control (2A3) (CL647-65209, Clone: 2A3) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Tested Applications

Positive IF detected inNIH/3T3 cells
Positive FC detected inNIH/3T3 cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL647-65052 targets CD107b / LAMP2 in IF, FC (Intra) applications and shows reactivity with Mouse samples.

Tested Reactivity Mouse
Host / Isotype Rat / IgG2a, kappa
Class Monoclonal
Type Antibody
Immunogen Glycoproteins purified from BALB/c mouse embryo 3T3 cell line
Full Name lysosomal-associated membrane protein 2
GenBank Accession NumberBC138718
Gene Symbol CD107b
Gene ID (NCBI) 16784
RRIDAB_2883655
Conjugate CoraLite® Plus 647 Fluorescent Dye
Excitation/Emission Maxima Wavelengths654 nm / 674 nm
Form Liquid
Purification MethodAffinity purification
Storage Buffer PBS with 0.1% sodium azide and 0.5% BSA, pH 7.3.
Storage ConditionsStore at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

LAMP2 (CD107b) is a Lysosomal membrane glycoprotein. LAMP2 is extensively glycosylated with asparagine-linked oligosaccharides which protect it from intracellular proteolysis (PMID: 10521503). Although LAMP-2 is localized primarily in the endosome-lysosomal membrane of cells, it is also found on the plasma membrane under certain circumstances, e.g., after platelet activation, during granulocytic differentiation and activation, and in some tumor cells (PMID: 12221139). LAMP is involved in lysosomal stability and autophagy (PMID: 12221139). This glycoprotein provides selectins with carbohydrate ligands. LAMP2 may plays a role in tumor cell metastasis (PMID 9426697).

Protocols

Product Specific Protocols
IF protocol for CL Plus 647 CD107b / LAMP2 antibody CL647-65052Download protocol
Standard Protocols
Click here to view our Standard Protocols