CoraLite®555 Anti-Mouse CD107a / LAMP1 (1D4B)

CD107a / LAMP1 Monoclonal Antibody for IF/ICC, FC (Intra)

Host / Isotype

Rat / IgG2a, kappa

Reactivity

Mouse

Applications

IF/ICC, FC (Intra)

Conjugate

CoraLite®555 Fluorescent Dye

CloneNo.

1D4B

Cat no : CL555-65050

Synonyms

AI196048, CD107a, Lamp 1, Lamp1

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Validation Data Gallery

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  IF Staining of NIH/3T3 using CL555-65050

  Immunofluorescent analysis of (-20°C Ethanol) fixed NIH/3T3 cells using CoraLite®555 CD107a antibody (CL555-65050, Clone: 1D4B ) at dilution of 1:100.

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  IF Staining of NIH/3T3 using CL555-65050

  Immunofluorescent analysis of (-20°C Ethanol) fixed NIH/3T3 cells using CoraLite®555 CD107a antibody (CL555-65050, Clone: 1D4B ) at dilution of 1:100.

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  FC experiment of NIH/3T3 using CL555-65050

  1x10^6 NIH/3T3 cells were intracellularly stained with 0.5 ug CoraLite®555 Anti-Mouse CD107a / LAMP1 (CL555-65050, Clone:1D4B) (red), or 0.5 ug CoraLite® Plus 555 Rat IgG2a Isotype Control (2A3) (CL555-65209, Clone: 2A3) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

• Positive IF/ICC detected in: NIH/3T3 cells
• Positive FC (Intra) detected in: NIH/3T3 cells
• Positive FC detected in: NIH/3T3 cells

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL555-65050 targets CD107a / LAMP1 in IF/ICC, FC (Intra) applications and shows reactivity with Mouse samples.

• Tested Reactivity: Mouse
• Host / Isotype: Rat / IgG2a, kappa
• Class: Monoclonal
• Type: Antibody
• Immunogen: Plasma membrane fraction of mouse embryo NIH/3T3 cell line
• Full Name: lysosomal-associated membrane protein 1
• GenBank Accession Number: BC006785
• Gene Symbol: CD107a
• Gene ID (NCBI): 16783
• RRID: AB_2919664
• Conjugate: CoraLite®555 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 557 nm / 570 nm
• Form: Liquid
• Purification Method: Affinity purification
• Storage Buffer: PBS with 0.1% sodium azide and 0.5% BSA, pH 7.3.
• Storage Conditions: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

LAMP1 (CD107a) is a heavily glycosylated membrane protein enriched in the lysosomal membrane. LAMP1 is extensively glycosylated with asparagine-linked oligosaccharides which protect it from intracellular proteolysis (PMID: 10521503). Although LAMP1 is expressed largely in the endosome-lysosomal membrane of cells, it is also found on the plasma membrane (PMID: 16168398). Elevated LAMP1 expression at the cell surface has also been detected during platelet and granulocytic cell activation, as well as in some tumor cells (PMID: 29085473). LAMP1 functions to provide selectins with carbohydrate ligands. This protein has also been shown to be a marker of degranulation on lymphocytes such as CD8+ and NK cells and may also play a role in tumor cell differentiation and metastasis (PMID: 18835598; 29085473; 9426697).

Related Products

Flow Cytometry Perm Buffer (10X)

PF00011-C