CoraLite® Plus 405 Anti-Mouse CD107a / LAMP1 (1D4B)

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  FC experiment of NIH/3T3 using CL405-65050

  1x10^6 NIH/3T3 cells were intracellularly stained with 0.5 ug CoraLite® Plus 405 Anti-Mouse CD107a / LAMP1 (CL405-65050, Clone: 1D4B) (red), or 0.5 ug CoraLite® Plus 405 Rat IgG2a Isotype Control (2A3) (CL405-65209, Clone: 2A3) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

CD107a / LAMP1 Monoclonal Antibody for FC (Intra)

Cat No. CL405-65050

Clone No.1D4B

Host / Isotype

Rat / IgG2a, kappa

Reactivity

Mouse

Applications

FC (Intra)

Synonyms

AI196048, CD107a, Lamp 1, Lamp1

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Tested Applications

• Positive FC (Intra) detected in: NIH/3T3 cells
• Positive FC detected in: NIH/3T3 cells

Recommended dilution

• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.5 ug per 10^6 cells in 100 μl suspension
• Flow Cytometry (FC): FC :

This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL405-65050 targets CD107a / LAMP1 in FC (Intra) applications and shows reactivity with Mouse samples.

• Tested Reactivity: Mouse
• Host / Isotype: Rat / IgG2a, kappa
• Class: Monoclonal
• Type: Antibody
• Immunogen: Plasma membrane fraction of mouse embryo NIH/3T3 cell line
• Full Name: lysosomal-associated membrane protein 1
• GenBank Accession Number: BC006785
• Gene Symbol: CD107a
• Gene ID (NCBI): 16783
• RRID: AB_3672381
• Conjugate: CoraLite® Plus 405 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 399 nm / 422 nm
• Form: Liquid
• Purification Method: Affinity purification
• UNIPROT ID: P11438
• Storage Buffer: PBS with 0.09% sodium azide, pH 7.3.
• Storage Conditions: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

LAMP1 (CD107a) is a heavily glycosylated membrane protein enriched in the lysosomal membrane. LAMP1 is extensively glycosylated with asparagine-linked oligosaccharides which protect it from intracellular proteolysis (PMID: 10521503). Although LAMP1 is expressed largely in the endosome-lysosomal membrane of cells, it is also found on the plasma membrane (PMID: 16168398). Elevated LAMP1 expression at the cell surface has also been detected during platelet and granulocytic cell activation, as well as in some tumor cells (PMID: 29085473). LAMP1 functions to provide selectins with carbohydrate ligands. This protein has also been shown to be a marker of degranulation on lymphocytes such as CD8+ and NK cells and may also play a role in tumor cell differentiation and metastasis (PMID: 18835598; 29085473; 9426697).