CCT6B Monoclonal antibody, PBS Only

CCT6B Monoclonal Antibody for WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2F11A9

Cat no : 67961-1-PBS

Synonyms

Chaperonin containing T-complex polypeptide 1 subunit 6B, CCT-zeta-like, CCT-zeta-2, Cctz2, CCTZ 2

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Validation Data Gallery

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  WB analysis using 67961-1-Ig (same clone as 67961-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67961-1-Ig (CCT6B antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67961-1-PBS in a different storage buffer formulation.

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  WB analysis of NIH/3T3 using 67961-1-Ig (same clone as 67961-1-PBS)

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 67961-1-Ig (CCT6B antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67961-1-PBS in a different storage buffer formulation.

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  WB analysis of HSC-T6 using 67961-1-Ig (same clone as 67961-1-PBS)

  HSC-T6 cells were subjected to SDS PAGE followed by western blot with 67961-1-Ig (CCT6B antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67961-1-PBS in a different storage buffer formulation.

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  WB analysis of HEK-293 using 67961-1-Ig (same clone as 67961-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 67961-1-Ig (CCT6B antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67961-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse testis using 67961-1-Ig (same clone as 67961-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 67961-1-Ig (CCT6B antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67961-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse testis using 67961-1-Ig (same clone as 67961-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 67961-1-Ig (CCT6B antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67961-1-PBS in a different storage buffer formulation.

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  IF Staining of U2OS using 67961-1-Ig (same clone as 67961-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed U2OS cells using CCT6B antibody (67961-1-Ig, Clone: 2F11A9 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 67961-1-PBS in a different storage buffer formulation.

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  FC experiment of U2OS using 67961-1-Ig (same clone as 67961-1-PBS)

  1X10^6 U2OS cells were intracellularly stained with 0.4 ug Anti-Human CCT6B (67961-1-Ig, Clone:2F11A9) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 67961-1-PBS in a different storage buffer formulation.

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Product Information

67961-1-PBS targets CCT6B in WB, IHC, IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: CCT6B fusion protein Ag31295
• Full Name: chaperonin containing TCP1, subunit 6B (zeta 2)
• Calculated Molecular Weight: 530 aa, 58 kDa
• Observed Molecular Weight: 53-58 kDa
• GenBank Accession Number: BC027591
• Gene Symbol: CCT6B
• Gene ID (NCBI): 10693
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

CCT6B, also named as CCT-zeta-2, TCP-1-zeta-like, CCT-zeta-like, Testis-specific Tcp20 and Testis-specific protein TSA303, belongs to the TCP-1 chaperonin family. CCT6B is a molecular chaperone which assists the folding of proteins upon ATP hydrolysis. It is known to play a role, in vitro, in the folding of actin and tubulin. CCT6B has 3 isoforms with the molecular mass of 53-58 kDa.