CCT6B Monoclonal antibody

CCT6B Monoclonal Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA
Cat No. 67961-1-Ig
Clone No.2F11A9

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, FC (Intra), ELISA

Chaperonin containing T-complex polypeptide 1 subunit 6B, CCT-zeta-like, CCT-zeta-2, Cctz2, CCTZ 2

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
CoraLite® Plus 488
Size/Concentration: 

-/ -

Freight/Packing: -

Quantity

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Tested Applications

Positive WB detected inJurkat cells, NIH/3T3 cells, HSC-T6 cells, HEK-293 cells, K-562 cells
Positive IHC detected inmouse testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inU2OS cells
Positive FC (Intra) detected inU2OS cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

67961-1-Ig targets CCT6B in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen CCT6B fusion protein Ag31295 Predict reactive species
Full Name chaperonin containing TCP1, subunit 6B (zeta 2)
Calculated Molecular Weight 530 aa, 58 kDa
Observed Molecular Weight53-58 kDa
GenBank Accession NumberBC027591
Gene Symbol CCT6B
Gene ID (NCBI) 10693
RRIDAB_2918712
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ92526
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CCT6B, also named as CCT-zeta-2, TCP-1-zeta-like, CCT-zeta-like, Testis-specific Tcp20 and Testis-specific protein TSA303, belongs to the TCP-1 chaperonin family. CCT6B is a molecular chaperone which assists the folding of proteins upon ATP hydrolysis. It is known to play a role, in vitro, in the folding of actin and tubulin. CCT6B has 3 isoforms with the molecular mass of 53-58 kDa.

Protocols

Product Specific Protocols
WB protocol for CCT6B antibody 67961-1-IgDownload protocol
IHC protocol for CCT6B antibody 67961-1-IgDownload protocol
IF protocol for CCT6B antibody 67961-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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