CCKAR-specific Recombinant antibody, PBS Only (Detector)

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  WB analysis using 85205-1-RR (same clone as 85205-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 85205-1-RR (CCKAR-specific antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85205-1-PBS in a different storage buffer formulation.

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  Sandwich ELISA standard curve of MP01958-1

  Sandwich ELISA standard curve of MP01958-1, Human CCKAR Recombinant Matched Antibody Pair - PBS only. 85205-2-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard SY00105. 85205-1-PBS was HRP conjugated as the detection antibody. Range: 78.1-5000 pg/mL.

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  Sample test of MP01958-1

  The mean CCKAR concentration was determined to be 4.0 ng/mL in HUVEC cell extract based on a 0.9 mg/mL extract load and 5.3 ng/mL in SGC-7901 cell extract based on a 2.4 mg/mL extract load.

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  IP experiment of mouse lung using 85205-1-RR (same clone as 85205-1-PBS)

  IP result of anti-CCKAR-specific (IP:85205-1-RR, 4ug; Detection:85205-1-RR 1:600) with mouse lung tissue lysate 1520 ug. This data was developed using the same antibody clone with 85205-1-PBS in a different storage buffer formulation.

CCKAR-specific {ptg:Brand} Recombinant Antibody for WB, IP, Sandwich ELISA, Indirect ELISA, Sample test

Cat No. 85205-1-PBS

Clone No.242681A12

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, Sandwich ELISA, Indirect ELISA, Sample test

Synonyms

CCKAR, CCK1-R, CCK1 R, CCK AR, CCK A receptor

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Product Information

85205-1-PBS targets CCKAR-specific as part of a matched antibody pair:

MP01958-1: 85205-2-PBS capture and 85205-1-PBS detection (validated in Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Peptide
• Full Name: cholecystokinin A receptor
• Calculated Molecular Weight: 48 kDa
• Observed Molecular Weight: 75 kDa
• GenBank Accession Number: NM_000730
• Gene Symbol: CCKAR
• Gene ID (NCBI): 886
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P32238
• Storage Buffer: PBS only , pH 7.3
• Storage Conditions: Store at -80°C.

Background Information

Cholecystokinin (CCK) receptor, including CCKAR and CCKBR, is a receptor for cholecystokinin, mainly regulating pancreatic growth and enzyme secretion, and smooth muscle contraction of the gallbladder and stomach (PMID: 34556863). CCKAR (Cholecystokinin receptor type A) is primarily expressed in the alimentary tract, while CCKBR is mainly found in the brain and the stomach (PMID: 16816139). CCKAR is a biomarker for prognosis and asynchronous brain metastasis of non-small cell lung cancer (PMID: 36733361). The calculated MW of CCKAR is 48 kDa, but the observed MW is around 75 kDa, which may be with glycosylation modification.