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  • Western Blot (WB) analysis of various lysates using CCKAR-specific Recombinant antibody (85205-1-RR)

    WB analysis using 85205-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 85205-1-RR (CCKAR-specific antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of mouse lung tissue using CCKAR-specific Recombinant antibody (85205-1-RR)

    IP experiment of mouse lung using 85205-1-RR

    IP result of anti-CCKAR-specific (IP:85205-1-RR, 4ug; Detection:85205-1-RR 1:600) with mouse lung tissue lysate 1520 ug.

CCKAR-specific Recombinant antibody

CCKAR-specific {ptg:Brand} Recombinant Antibody for WB, IP, ELISA
Cat No. 85205-1-RR
Clone No.242681A12

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, ELISA

Synonyms

CCKAR, CCK1-R, CCK1 R, CCK AR, CCK A receptor

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

Freight/Packing: -

Quantity

- +

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Tested Applications

Positive WB detected inSGC-7901 cells, mouse stomach tissue, rat stomach tissue, mouse pancreas tissue, rat pancreas tissue, mouse bladder tissue, mouse lung tissue
Positive IP detected inmouse lung tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

85205-1-RR targets CCKAR-specific in WB, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Peptide Predict reactive species
Full Name cholecystokinin A receptor
Calculated Molecular Weight 48 kDa
Observed Molecular Weight75 kDa
GenBank Accession NumberNM_000730
Gene Symbol CCKAR
Gene ID (NCBI) 886
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP32238
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Cholecystokinin (CCK) receptor, including CCKAR and CCKBR, is a receptor for cholecystokinin, mainly regulating pancreatic growth and enzyme secretion, and smooth muscle contraction of the gallbladder and stomach (PMID: 34556863). CCKAR (Cholecystokinin receptor type A) is primarily expressed in the alimentary tract, while CCKBR is mainly found in the brain and the stomach (PMID: 16816139). CCKAR is a biomarker for prognosis and asynchronous brain metastasis of non-small cell lung cancer (PMID: 36733361). The calculated MW of CCKAR is 48 kDa, but the observed MW is around 75 kDa, which may be with glycosylation modification.

Protocols

Product Specific Protocols
WB protocol for CCKAR-specific antibody 85205-1-RRDownload protocol
IP protocol for CCKAR-specific antibody 85205-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols