CoraLite® Plus 488-conjugated CCDC94 Recombinant antibody

CCDC94 Uni-rAb^TM Recombinant Antibody for IF/ICC, FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

240517E9

Cat no : CL488-83580-5

Synonyms

YJU2, Coiled-coil domain-containing protein 94, 240517E9

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Validation Data Gallery

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  IF Staining of A549 using CL488-83580-5

  Immunofluorescent analysis of (4% PFA) fixed A549 cells using CoraLite® Plus 488 CCDC94 antibody (CL488-83580-5, Clone: 240517E9 ) at dilution of 1:200.

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  FC experiment of A549 using CL488-83580-5

  1x10^6 A549 cells were intracellularly stained with 0.25 ug CoraLite® Plus 488-conjugated CCDC94 Recombinant antibody (CL488-83580-5, Clone:240517E9)(red), or 0.25 ug CoraLite® Plus 488 Rabbit IgG Isotype Control Recombinant Antibody (CL488-98136, Clone: 240953C9) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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Tested Applications

• Positive IF/ICC detected in: A549 cells
• Positive FC (Intra) detected in: A549 cells

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of CL488-83580-5 is CCDC94 Fusion Protein expressed in E. coli.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: CCDC94 fusion protein Ag13764
• Full Name: coiled-coil domain containing 94
• Calculated Molecular Weight: 323 aa, 37 kDa
• GenBank Accession Number: BC000561
• Gene Symbol: CCDC94
• Gene ID (NCBI): 55702
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

CCDC94, also known as YJU2, is a splicing factor, predicted to be involved in RNA splicing and negative regulation of DNA damage response, signal transduction by p53 class mediator. Part of U2-type catalytic step 1 spliceosome. And that it is required for pre-mRNA splicing both in vivo and in vitro. Yju2 acts in concert with an unidentified heat-resistant factor(s) in an ATP-independent manner to promote the first catalytic reaction of pre-mRNA splicing after Prp2-mediated structural rearrangement of the spliceosome. (PMID: 17515604)