CBX5 Recombinant antibody, PBS Only

CBX5 Uni-rAb^TM Recombinant Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

240145G12

Cat no : 83258-6-PBS

Synonyms

Antigen p25, CBX5, Chromobox protein homolog 5, HP1, HP1 alpha, HP1A

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Validation Data Gallery

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  WB analysis using 83258-6-RR (same clone as 83258-6-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 83258-6-RR (CBX5 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83258-6-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 83258-6-RR (same clone as 83258-6-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CBX5 antibody (83258-6-RR, Clone: 240145G12 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-Phalloidin (red). This data was developed using the same antibody clone with 83258-6-PBS in a different storage buffer formulation.

• 

  IF Staining of HepG2 using 83258-6-RR (same clone as 83258-6-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CBX5 antibody (83258-6-RR, Clone: 240145G12 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-Phalloidin (red). This data was developed using the same antibody clone with 83258-6-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 83258-6-PBS

  Biolayer interferometry (BLl) kinetic assays of 83258-6-RR against Human CBX5 were performed. The affinity constant is 0.606 nM.

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Product Information

The immunogen of 83258-6-PBS is CBX5 Fusion Protein expressed in E. coli.

• Tested Reactivity: Human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: CBX5 fusion protein Ag34810
• Full Name: chromobox homolog 5 (HP1 alpha homolog, Drosophila)
• Calculated Molecular Weight: 191 aa, 22 kDa
• GenBank Accession Number: BC006821
• Gene Symbol: CBX5
• Gene ID (NCBI): 23468
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.