CBX5 Recombinant antibody

CBX5 Uni-rAb^TM Recombinant Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

240145G12

Cat no : 83258-6-RR

Synonyms

Antigen p25, CBX5, Chromobox protein homolog 5, HP1, HP1 alpha, HP1A

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Validation Data Gallery

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  WB analysis using 83258-6-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 83258-6-RR (CBX5 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IF Staining of HepG2 using 83258-6-RR

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CBX5 antibody (83258-6-RR, Clone: 240145G12 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-Phalloidin (red).

• 

  IF Staining of HepG2 using 83258-6-RR

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CBX5 antibody (83258-6-RR, Clone: 240145G12 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-Phalloidin (red).

• 

  Affinity and Kinetic Characterization of 83258-6-RR

  Biolayer interferometry (BLl) kinetic assays of 83258-6-RR against Human CBX5 were performed. The affinity constant is 0.606 nM.

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Tested Applications

• Positive WB detected in: HeLa cells, HEK-293 cells, MCF-7 cells, Jurkat cells, A431 cells
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 83258-6-RR is CBX5 Fusion Protein expressed in E. coli.

• Tested Reactivity: Human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: CBX5 fusion protein Ag34810
• Full Name: chromobox homolog 5 (HP1 alpha homolog, Drosophila)
• Calculated Molecular Weight: 191 aa, 22 kDa
• Observed Molecular Weight: 24 kDa
• GenBank Accession Number: BC006821
• Gene Symbol: CBX5
• Gene ID (NCBI): 23468
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Chromobox protein homolog 5 (CBX5), also named heterochromatin protein 1 alpha (HP1a), is a highly conserved nonhistone protein involved in heterochromatin formation and gene silencing in different species including humans.HP1a is a Component of heterochromatin that recognizes and binds histone H3 tails methylated at 'Lys-9' (H3K9me), leading to epigenetic repression. In contrast, it is excluded from chromatin when 'Tyr-41' of histone H3 is phosphorylated (H3Y41ph). It may interact with lamin-Breceptor. HP1a is involved in the formation of functional kinetochore through interaction with MIS12complex proteins. Phosphorylation of HP1 and LBR during interphase mitosis may be responsible for some of the alterations in chromatin organization and nuclear structure which occur at various times during the cell cycle.The HP1a was expressed in nucleus and associates specifically with chromatin during metaphase and anaphase.Recent studies have shown that HP1a is present at many euchromatic sites and positively regulates euchromatic gene expression through RNA transcript association and interaction with hnRNPs in Drosophila (19798443).