CoraLite® Plus 488-conjugated CBS Polyclonal antibody

• Featured Product
• KD/KO Validated

CBS Polyclonal Antibody for IF/ICC

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF/ICC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat no : CL488-14787

Synonyms

Beta thionase, CBS, cystathionine beta synthase, HIP4, Serine sulfhydrase

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Validation Data Gallery

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  IF Staining of HeLa using CL488-14787

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CoraLite® Plus 488 CBS antibody (CL488-14787) at dilution of 1:200, CL594-Phalloidin (red).

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  IF Staining of HeLa using CL488-14787

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using CoraLite® Plus 488 CBS antibody (CL488-14787) at dilution of 1:200.

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Tested Applications

• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL488-14787 targets CBS in IF/ICC applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CBS fusion protein Ag6437
• Full Name: cystathionine-beta-synthase
• Calculated Molecular Weight: 61 kDa
• Observed Molecular Weight: 61-63 kDa
• GenBank Accession Number: BC000440
• Gene Symbol: CBS
• Gene ID (NCBI): 875
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

The CBS gene encodes cystathionine beta-synthase, which catalyzes the first irreversible step of transsulfuration. The CBS enzyme is a homotetramer of 63-kD subunits and requires pyridoxal phosphate and heme for activity(PMID:11230183). CBS protein is localized in most areas of the brain, but predominantly in the cell bodies and neuronal processes of Purkinje cells and Ammon's horn neurons(PMID:12588964).