c-Cbl Monoclonal antibody, PBS Only

• Featured Product
• KD/KO Validated

c-Cbl Monoclonal Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2F6D1

Cat no : 66576-1-PBS

Synonyms

C CBL, CBL, CBL2, c-Cbl, Proto oncogene c Cbl, RING finger protein 55, RNF55

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Validation Data Gallery

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  WB analysis using 66576-1-Ig (same clone as 66576-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66576-1-Ig (c-Cbl antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66576-1-PBS in a different storage buffer formulation.

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  WB analysis of Daudi using 66576-1-Ig (same clone as 66576-1-PBS)

  Daudi cells were subjected to SDS PAGE followed by western blot with 66576-1-Ig (CBL antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66576-1-PBS in a different storage buffer formulation.

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  IHC staining of human tonsillitis using 66576-1-Ig (same clone as 66576-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66576-1-Ig (CBL antibody) at dilution of 1:600 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66576-1-PBS in a different storage buffer formulation.

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  IHC staining of human tonsillitis using 66576-1-Ig (same clone as 66576-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66576-1-Ig (CBL antibody) at dilution of 1:600 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66576-1-PBS in a different storage buffer formulation.

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Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66576-1-PBS targets c-Cbl in WB, IHC, Indirect ELISA applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: c-Cbl fusion protein Ag23033
• Full Name: Cas-Br-M (murine) ecotropic retroviral transforming sequence
• Calculated Molecular Weight: 906 aa, 100 kDa
• Observed Molecular Weight: 100-120 kDa
• GenBank Accession Number: BC132733
• Gene Symbol: CBL
• Gene ID (NCBI): 867
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

c-Cbl (Casitas B-lineage lymphoma) is a critical regulator that controls immature or follicular helper T cell development, B cell development, immune tolerance, angiogenesis, or tumorigenesis (PMID: 33962939). c-Cbl also plays a pivotal role in regulating dendritic cell (DC) function via negatively modulating IL-12 production after Toll-like receptor (TLR) stimulation (PMID: 21799517).