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CoraLite® Plus 488-conjugated CBFB Monoclonal antibody

CBFB Monoclonal Antibody for FC (Intra)

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1D7F2

Cat no : CL488-67885

Synonyms

PEBP2 beta, PEA2-beta, Core-binding factor subunit beta, CBF-beta, CBF beta

Validation Data Gallery

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  • Flow cytometry (FC) experiment of K-562 cells using CoraLite® Plus 488-conjugated CBFB Monoclonal anti (CL488-67885)

    FC experiment of K-562 using CL488-67885

    1X10^6 K-562 cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human CBFB (CL488-67885, Clone:1D7F2) (red), or 0.4 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

Tested Applications

Positive FC (Intra) detected inK-562 cells

Recommended dilution

ApplicationDilution
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-67885 targets CBFB in FC (Intra) applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen CBFB fusion protein Ag31183
Full Name core-binding factor, beta subunit
Calculated Molecular Weight 182 aa, 22 kDa
GenBank Accession NumberBC018509
Gene Symbol CBFB
Gene ID (NCBI) 865
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CBFB (core binding factor subunit β), forms the heterodimeric complex core-binding factor (CBF). As a critical transcription factor, CBFB is frequently mutated in breast cancer and considered to be of significance in the pathogenesis of cancer.(PMID: 29932212) The heterodimers bind to the core site of a number of enhancers and promoters, including murine leukemia virus, polyomavirus enhancer, T-cell receptor enhancers, LCK, IL3 and GM-CSF promoters.) It has calculated molecular weight around 22kDa.

Protocols

Product Specific Protocols
FC protocol for CL Plus 488 CBFB antibody CL488-67885Download protocol
Standard Protocols
Click here to view our Standard Protocols