Filter: AllWBIPIHCIF/ICC

WB analysis of Jurkat using 66093-1-Ig (same clone as 66093-1-PBS)
Untreated and Staurosporine treated Jurkat cells were subjected to SDS PAGE followed by western blot with 66093-1-Ig (Caspase 8 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66093-1-PBS in a different storage buffer formulation.
WB analysis of HeLa cells using 66093-1-Ig (same clone as 66093-1-PBS)
WB result of CASP8 with normal and apotosis Hela cell. P18 can be get in the apotosis cell. 60kd,50kd and 45kd bands are some isoforms of precursor CASP8. This data was developed using the same antibody clone with 66093-1-PBS in a different storage buffer formulation.
WB analysis using 66093-1-Ig (same clone as 66093-1-PBS)
Various lysates were subjected to SDS PAGE followed by western blot with 66093-1-Ig (Caspase 8 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66093-1-PBS in a different storage buffer formulation.
WB analysis of HepG2 using 66093-1-Ig (same clone as 66093-1-PBS)
HepG2 cells were subjected to SDS PAGE followed by western blot with 66093-1-Ig (CASP8 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66093-1-PBS in a different storage buffer formulation.
WB analysis of HeLa using 66093-1-Ig (same clone as 66093-1-PBS)
HeLa cells were subjected to SDS PAGE followed by western blot with 66093-1-Ig (CASP8 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66093-1-PBS in a different storage buffer formulation.
IP experiment of HepG2 using 66093-1-Ig (same clone as 66093-1-PBS)
IP result of anti-Caspase 8/p43/p18 (IP:66093-1-Ig, 5ug; Detection:66093-1-Ig 1:500) with HepG2 cells lysate 2400ug. This data was developed using the same antibody clone with 66093-1-PBS in a different storage buffer formulation.
IHC staining of human liver cancer using 66093-1-Ig (same clone as 66093-1-PBS)
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66093-1-Ig (Caspase 8 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66093-1-PBS in a different storage buffer formulation.
IHC staining of human liver cancer using 66093-1-Ig (same clone as 66093-1-PBS)
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66093-1-Ig (Caspase 8 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66093-1-PBS in a different storage buffer formulation.
IHC staining of human liver using 66093-1-Ig (same clone as 66093-1-PBS)
Immunohistochemical analysis of paraffin-embedded human liver slide using 66093-1-Ig (CASP8 Antibody) at dilution of 1:50. This data was developed using the same antibody clone with 66093-1-PBS in a different storage buffer formulation.
IHC staining of human liver using 66093-1-Ig (same clone as 66093-1-PBS)
Immunohistochemical analysis of paraffin-embedded human liver slide using 66093-1-Ig (CASP8 Antibody) at dilution of 1:50. This data was developed using the same antibody clone with 66093-1-PBS in a different storage buffer formulation.
IF Staining of HeLa using 66093-1-Ig (same clone as 66093-1-PBS)
Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using Caspase 8/p43/p18 antibody (66093-1-Ig, Clone: 2B9H8 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 66093-1-PBS in a different storage buffer formulation.

Featured Product
KD/KO Validated

Caspase 8/p43/p18 Monoclonal antibody, PBS Only

Caspase 8/p43/p18 Monoclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA

Cat No. 66093-1-PBS

Review Product

Host / Isotype

Mouse / IgG2b

Reactivity

human

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Synonyms

CASP8, Caspase 8, CASP-8, CAP4, Apoptotic protease Mch-5

Formulation: PBS Only

PBS and Azide

PBS Only

Conjugate: Unconjugated

Size/Concentration:

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Freight/Packing: -

Quantity

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Overview

Tested Applications
Product Information

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MSDS SDS

Product Information

66093-1-PBS targets Caspase 8/p43/p18 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity	human
Host / Isotype	Mouse / IgG2b
Class	Monoclonal
Type	Antibody
Immunogen	Caspase 8/p43/p18 fusion protein Ag20524 Predict reactive species
Full Name	caspase 8, apoptosis-related cysteine peptidase
Calculated Molecular Weight	538 aa, 62 kDa
Observed Molecular Weight	53-57 kDa, 32-45 kDa, 18 kDa
GenBank Accession Number	BC028223
Gene Symbol	Caspase 8
Gene ID (NCBI)	841
RRID	AB_11232214
Conjugate	Unconjugated
Form	Liquid
Purification Method	Protein A purification
UNIPROT ID	Q14790
Storage Buffer	PBS Only
Storage Conditions	Store at -80°C.

Background Information

Caspase 8, also named as MCH5, MACH, FLICE, and CAP4, belongs to the peptidase C14A family. It may participate in the GZMB apoptotic pathways and functions as an upstream regulator in a-bisabolol-induced apoptosis. Caspase 8 catalyzes an essential intermediate step in the ubiquitination and proteasome-mediated degradation of IRF3 (PMID:21816816). It may control diabetic embryopathy-associated apoptosis via regulation of the Bid-stimulated mitochondrion/caspase-9 pathway (PMID:19194987). Caspase 8 is expressed as nine isoforms by alternative splicing with the molecular mass from 26 kDa to 62 kDa. This antibody can recognize pro- and cleaved-caspase 8.