CoraLite® Plus 488-conjugated CALD1 Monoclonal antibody

• Featured Product
• KD/KO Validated

CALD1 Monoclonal Antibody for IF/ICC, FC (Intra)

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse, rat

Applications

IF/ICC, FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1E1C11

Cat no : CL488-66693

Synonyms

Caldesmon, CDM, caldesmon 1, CAD, 1E1C11

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Validation Data Gallery

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  IF Staining of HepG2 using CL488-66693

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CL488-66693 (CALD1 antibody) at dilution of 1:100.

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  FC experiment of HeLa using CL488-66693

  1X10^6 HeLa cells were intracellularly stained with 0.8 ug CoraLite® Plus 488 Anti-Human CALD1 (CL488-66693, Clone:1E1C11) (red), or 0.8 ug CoraLite® Plus 488 Mouse IgG2b Isotype Control (MPC-11) (CL488-65128, Clone: MPC-11) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

• Positive IF/ICC detected in: HepG2 cells
• Positive FC (Intra) detected in: HeLa cells

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL488-66693 targets CALD1 in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen: CALD1 fusion protein Ag13676
• Full Name: caldesmon 1
• Calculated Molecular Weight: 538 aa, 63 kDa
• Observed Molecular Weight: 70 kDa~75 kDa
• GenBank Accession Number: BC040354
• Gene Symbol: CALD1
• Gene ID (NCBI): 800
• RRID: AB_2883368
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.