CoraLite® Plus 488-conjugated CA3 Polyclonal antibody

• Featured Product
• KD/KO Validated

CA3 Polyclonal Antibody for IF-P

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF-P

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat no : CL488-15197

Synonyms

CA III, CA3, CAIII, Car3, Carbonate dehydratase III, Carbonic anhydrase 3, Carbonic anhydrase III

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Validation Data Gallery

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  IF Staining of mouse skeletal muscle using CL488-15197

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse skeletal muscle tissue using CoraLite® Plus 488 CA3 antibody (CL488-15197) at dilution of 1:200. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive IF-P detected in: mouse skeletal muscle tissue

Recommended dilution

• Immunofluorescence (IF)-P: IF-P : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL488-15197 targets CA3 in IF-P applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CA3 fusion protein Ag7344
• Full Name: carbonic anhydrase III, muscle specific
• Calculated Molecular Weight: 29 kDa
• Observed Molecular Weight: 30 kDa
• GenBank Accession Number: BC004897
• Gene Symbol: CA3
• Gene ID (NCBI): 761
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

Carbonic anhydrase III (CA3), which belongs to the alpha-carbonic anhydrase family, is a cytoplasmic enzyme that exhibits a relatively low carbon dioxide hydratase activity. It is expressed at a very high level in skeletal muscle, where physical exercise has been shown to increase free radical production. In addition to its carbon dioxide hydratase activity, CA3 has been demonstrated to have a carboxyl esterase activity and phosphatase activity, which suggests that it is a tyrosine phosphatase (PMID: 10064618). CA3 was found to be localized in Type-I muscle fibers and could be used as a marker for abnormal Type-I muscle fibers in several neuromuscular diseases (PMID: 6221502).