CA3 Polyclonal antibody

• Featured Product
• KD/KO Validated

CA3 Polyclonal Antibody for WB, IHC, IF-P, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF-P, ELISA

Conjugate

Unconjugated

Publications

8

Reviews (1)

Cat no : 15197-1-AP

Synonyms

Carbonic anhydrase III, Carbonate dehydratase III, Car3, CA-III, CAIII

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Validation Data Gallery

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  WB analysis using 15197-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15197-1-AP (CA3 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  WB analysis using 15197-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 15197-1-AP (CA3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis using 15197-1-AP

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 15197-1-AP (CA3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse skeletal muscle using 15197-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 15197-1-AP (CA3 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse skeletal muscle using 15197-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 15197-1-AP (CA3 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse skeletal muscle using 15197-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 15197-1-AP (CA3 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of human kidney using 15197-1-AP

  Immunofluorescent analysis of (4% PFA) fixed human kidney tissue using CA3 antibody (15197-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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  IF Staining of human kidney using 15197-1-AP

  Immunofluorescent analysis of (4% PFA) fixed human kidney tissue using CA3 antibody (15197-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of mouse skeletal muscle using 15197-1-AP

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse skeletal muscle tissue using CA3 antibody (15197-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: HEK-293 cells, mouse skeletal muscle tissue, rat skeletal muscle tissue, HeLa cells
• Positive IHC detected in: mouse skeletal muscle tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF-P detected in: mouse skeletal muscle tissue, human kidney tissue

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:16000
• Immunohistochemistry (IHC): IHC : 1:200-1:1000
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 3 publications below
• IHC: See 3 publications below
• IF: See 2 publications below

Product Information

15197-1-AP targets CA3 in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CA3 fusion protein Ag7344
• Full Name: carbonic anhydrase III, muscle specific
• Calculated Molecular Weight: 29 kDa
• Observed Molecular Weight: 30 kDa
• GenBank Accession Number: BC004897
• Gene Symbol: CA3
• Gene ID (NCBI): 761
• RRID: AB_2243603
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Carbonic anhydrase III (CA3), which belongs to the alpha-carbonic anhydrase family, is a cytoplasmic enzyme that exhibits a relatively low carbon dioxide hydratase activity. It is expressed at a very high level in skeletal muscle, where physical exercise has been shown to increase free radical production. In addition to its carbon dioxide hydratase activity, CA3 has been demonstrated to have a carboxyl esterase activity and phosphatase activity, which suggests that it is a tyrosine phosphatase (PMID: 10064618). CA3 was found to be localized in Type-I muscle fibers and could be used as a marker for abnormal Type-I muscle fibers in several neuromuscular diseases (PMID: 6221502).

Publications

Species	Application	Title
rat	WB,IHC	J Nutr Biochem; Postnatal high-fat diet sex-specifically exacerbates prenatal dexamethasone-induced hypertension: Mass spectrometry-based quantitative proteomic approach.; Authors - Chien-Ning Hsu
mouse	IF	Am J Physiol Renal Physiol; Studies of ultrastructure, gene expression, and marker analysis reveal that mouse bladder PDGFRA+ interstitial cells are fibroblasts; Authors - Dennis R. Clayton
		Clin Proteomics; Orthogonal proteomics methods warrant the development of Duchenne muscular dystrophy biomarkers; Authors - Camilla Johansson
mouse	WB	PLoS One; Multifactorial Comparative Proteomic Study of Cytochrome P450 2E1 Function in Chronic Alcohol Administration.; Authors - Yuan Wang ; KD Validated
mouse	IHC	PLoS One; Expression patterns and subcellular localization of carbonic anhydrases are developmentally regulated during tooth formation.; Authors - Claes-Göran Reibring
human,rat	WB	FEBS Open Bio; Carbonic anhydrase 3 increases during liver adipogenesis even in pre-obesity, and its inhibitors reduce liver adipose accumulation.; Authors - Hiroyuki Yamamoto

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Elise (Verified Customer) (06-21-2022)

Do not Work for IF with blocking serum 5% and antibody in BSA 1%. Will be tested on IHC and WB and Tested again in IF with 1: 150 dilution

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: kidney tissue