CoraLite® Plus 488-conjugated C7 Monoclonal antibody

C7 Monoclonal Antibody for IF/ICC, FC (Intra)

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

IF/ICC, FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1H9C4

Cat no : CL488-66908

Synonyms

Complement component C7, complement component 7, 1H9C4



Tested Applications

Positive IF/ICC detected inHepG2 cells
Positive FC (Intra) detected inHepG2 cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-66908 targets C7 in IF/ICC, FC (Intra) applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen C7 fusion protein Ag12552
Full Name complement component 7
Calculated Molecular Weight 843 aa, 94 kDa
Observed Molecular Weight 100 kDa
GenBank Accession NumberBC063851
Gene Symbol C7
Gene ID (NCBI) 730
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

C7, a single-chain plasma glycoprotein, is a component of the complement system. It is a constituent of the membrane attack complex (MAC) that plays a key role in the innate and adaptive immune response by forming pores in the plasma membrane of target cells. C7 serves as a membrane anchor. People with C7 deficiency are prone to bacterial infection.

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 C7 antibody CL488-66908Download protocol
FC protocol for CL Plus 488 C7 antibody CL488-66908Download protocol
Standard Protocols
Click here to view our Standard Protocols