CoraLite®594-conjugated C1QA Monoclonal antibody

C1QA Monoclonal Antibody for IF

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

IF/ICC

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

1B10E3

Cat no : CL594-67063

Synonyms

C1QA

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Validation Data Gallery

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  IF Staining of HepG2 using CL594-67063

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CoraLite®594 C1QA antibody (CL594-67063, Clone: 1B10E3 ) at dilution of 1:200, CL488-Phalloidin (green).

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Tested Applications

• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL594-67063 targets C1QA in IF/ICC applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: C1QA fusion protein Ag28622
• Full Name: complement component 1, q subcomponent, A chain
• Calculated Molecular Weight: 245 aa, 26 kDa
• Observed Molecular Weight: 30 kDa
• GenBank Accession Number: BC030153
• Gene Symbol: C1QA
• Gene ID (NCBI): 712
• RRID: AB_2920081
• Conjugate: CoraLite®594 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 588 nm / 604 nm
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Aliquoting is unnecessary for -20^oC storage.

Background Information

The first component of complement, C1, is a calcium-dependent complex of the 3 subcomponents C1q, C1r, and C1s. C1q is composed of 18 polypeptide chains: six A-chains, six B-chains, and six C-chains. Each chain contains a collagen-like region located near the N terminus and a C-terminal globular region. Deficiency of C1q has been associated with lupus erythematosus and glomerulonephritis. This antibody is raised against C1qA which is the A-chain polypeptide of human complement subcomponent C1q.