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ASUN Recombinant antibody, PBS Only (Capture)

ASUN Uni-rAbTM Recombinant Antibody for WB, IF/ICC, Cytometric bead array, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, Cytometric bead array, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

241586E9

Cat no : 84252-4-PBS

Synonyms

INTS13, Integrator complex subunit 13, Germ cell tumor 1, GCT1, C12orf11

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using ASUN Recombinant antibody (84252-4-RR)

    WB analysis using 84252-4-RR (same clone as 84252-4-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84252-4-RR (C12orf11 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84252-4-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using ASUN Recombinant antibody (84252-4-RR)

    IF Staining of HeLa using 84252-4-RR (same clone as 84252-4-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using ASUN antibody (84252-4-RR, Clone: 241586E9 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 84252-4-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP01142-1

    Cytometric bead array standard curve of MP01142-1

    Cytometric bead array standard curve of MP01142-1, C12orf11 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84252-4-PBS. Detection antibody: 84252-3-PBS. Standard: Ag13727. Range: 0.781-100 ng/mL.

Product Information

84252-4-PBS targets ASUN as part of a matched antibody pair:

MP01142-1: 84252-4-PBS capture and 84252-3-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen ASUN fusion protein Ag13727
Full Name chromosome 12 open reading frame 11
Calculated Molecular Weight 706 aa, 80 kDa
Observed Molecular Weight70-80 kDa
GenBank Accession NumberBC003081
Gene Symbol ASUN
Gene ID (NCBI) 55726
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Asunder (ASUN) is a conserved protein that has been reported as a critical regulator of dynein localization during Drosophila spermatogenesis (PMID: 19357193). Mammalian and Drosophila ASUN proteins revealed that they are 43% identical and 64% similar. Human ASUN (also known as GCT1 or C12ORF11) promotes dynein recruitment and centrosomal tethering to the nucleus at mitotic entry (PMID: 23097494).